Epigenetics & Nuclear Signaling Antibodies 4
Anti-Separin Antibody (CABP0469)
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- SKU:
- CABP0469
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IF
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-Separin Antibody |
| Antibody SKU: | CABP0469 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around S1126 of human ESPL1 (NP_036423.4). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:20 - 1:100 |
| Reactivity: | Human |
| Positive Samples: |
| Immunogen: | A synthetic phosphorylated peptide around S1126 of human ESPL1 (NP_036423.4). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | NSSP V |
| Gene ID: | 9700 |
| Uniprot: | Q14674 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 197kDa/233kDa |
| Observed MW: | Refer to Figures |
 | Immunofluorescence analysis of U2OS cells using Phospho-ESPL1-S1126 antibody (CABP0469). Blue: DAPI for nuclear staining. |
| Synonyms: | ESPL1, ESP1, SEPA, separin |
| Background: | Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM, Nov 2010] |
| UniProt Protein Function: | separase: a caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms. Regulated by at least two independent mechanisms. First, it is inactivated via its interaction with securin/PTTG1, which probably covers its active site. Second, phosphorylation inactivates it. It is completely phosphorylated during mitosis and dephosphorylated during anaphase. Two alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Protease; EC 3.4.22.49 Chromosomal Location of Human Ortholog: 12q Cellular Component: centrosome; nucleus; cytosol Molecular Function:protein binding; catalytic activity; cysteine-type peptidase activity Biological Process: establishment of mitotic spindle localization; homologous chromosome segregation; mitotic sister chromatid segregation; apoptosis; negative regulation of sister chromatid cohesion; positive regulation of mitotic metaphase/anaphase transition; cytokinesis; mitotic cell cycle; proteolysis; meiotic spindle organization and biogenesis |
| NCBI Summary: | Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM, Nov 2010] |
| UniProt Code: | Q14674 |
| NCBI GenInfo Identifier: | 308153600 |
| NCBI Gene ID: | 9700 |
| NCBI Accession: | Q14674.3 |
| UniProt Related Accession: | Q14674 |
| Molecular Weight: | 2120 |
| NCBI Full Name: | Separin |
| NCBI Synonym Full Names: | extra spindle pole bodies homolog 1 (S. cerevisiae) |
| NCBI Official Symbol: | ESPL1 |
| NCBI Official Synonym Symbols: | ESP1; SEPA |
| NCBI Protein Information: | separin; caspase-like protein ESPL1; extra spindle poles like 1; separin, cysteine protease; extra spindle poles-like 1 protein |
| UniProt Protein Name: | Separin |
| UniProt Synonym Protein Names: | Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase |
| Protein Family: | Separin |
| UniProt Gene Name: | ESPL1 |
| UniProt Entry Name: | ESPL1_HUMAN |
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