Cell Biology Antibodies 9
Anti-SEMG1 Antibody (CAB5487)
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- SKU:
- CAB5487
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-SEMG1 Antibody |
| Antibody SKU: | CAB5487 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 24-310 of human SEMG1 (NP_002998.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | THP-1, HT-1080 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 24-310 of human SEMG1 (NP_002998.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QKGG SKGR LPSE FSQF PHGQ KGQH YSGQ KGKQ QTES KGSF SIQY TYHV DAND HDQS RKSQ QYDL NALH KTTK SQRH LGGS QQLL HNKQ EGRD HDKS KGHF HRVV IHHK GGKA HRGT QNPS QDQG NSPS GKGI SSQY SNTE ERLW VHGL SKEQ TSVS GAQK GRKQ GGSQ SSYV LQTE ELVA NKQQ RETK NSHQ NKGH YQNV VEVR EEHS SKVQ TSLC PAHQ DKLQ HGSK DIFS TQDE LLVY NKNQ HQTK NLNQ DQQH GRKA NKIS YQSS STEE RRLH YGEN GVQK DVS |
| Gene ID: | 6406 |
| Uniprot: | P04279 |
| Cellular Location: | Secreted |
| Calculated MW: | 45kDa/52kDa |
| Observed MW: | 60kDa |
| Synonyms: | SEMG1, CT103, SEMG, SGI, dJ172H20.2 |
| Background: | The protein encoded by this gene is the predominant protein in semen. The encoded secreted protein is involved in the formation of a gel matrix that encases ejaculated spermatozoa. This preproprotein is proteolytically processed by the prostate-specific antigen (PSA) protease to generate multiple peptide products that exhibit distinct functions. One of these peptides, SgI-29, is an antimicrobial peptide with antibacterial activity. This proteolysis process also breaks down the gel matrix and allows the spermatozoa to move more freely. This gene and another similar semenogelin gene are present in a gene cluster on chromosome 20. |
| UniProt Protein Function: | SEMG1: Predominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA. Belongs to the semenogelin family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Cancer Testis Antigen (CTA); Secreted Chromosomal Location of Human Ortholog: 20q12-q13.2 Cellular Component: extracellular region; extracellular space; nucleus; protein complex Molecular Function:metal ion binding; protein binding Biological Process: antibacterial humoral response; cellular protein metabolic process; coagulation; insemination; protein heterooligomerization |
| NCBI Summary: | The protein encoded by this gene is the predominant protein in semen. The encoded secreted protein is involved in the formation of a gel matrix that encases ejaculated spermatozoa. This preproprotein is proteolytically processed by the prostate-specific antigen (PSA) protease to generate multiple peptide products that exhibit distinct functions. One of these peptides, SgI-29, is an antimicrobial peptide with antibacterial activity. This proteolysis process also breaks down the gel matrix and allows the spermatozoa to move more freely. This gene and another similar semenogelin gene are present in a gene cluster on chromosome 20. [provided by RefSeq, Feb 2016] |
| UniProt Code: | P04279 |
| NCBI GenInfo Identifier: | 134426 |
| NCBI Gene ID: | 6406 |
| NCBI Accession: | P04279.2 |
| UniProt Secondary Accession: | P04279,Q53ZV0, Q53ZV1, Q53ZV2, Q6X4I9, Q6Y809, Q6Y822 Q6Y823, Q86U64, Q96QM3, |
| UniProt Related Accession: | P04279 |
| Molecular Weight: | 45,322 Da |
| NCBI Full Name: | Semenogelin-1 |
| NCBI Synonym Full Names: | semenogelin I |
| NCBI Official Symbol: | SEMG1 |
| NCBI Official Synonym Symbols: | SGI; SEMG; CT103; dJ172H20.2 |
| NCBI Protein Information: | semenogelin-1 |
| UniProt Protein Name: | Semenogelin-1 |
| UniProt Synonym Protein Names: | Cancer/testis antigen 103; Semenogelin I; SGI |
| Protein Family: | Semenogelin |
| UniProt Gene Name: | SEMG1 |
| UniProt Entry Name: | SEMG1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SEMG1 antibody (CAB5487) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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