Cell Biology Antibodies 2

Anti-SEMA3F Antibody (CAB11713)

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SKU:
CAB11713
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-SEMA3F Antibody
Antibody SKU:CAB11713
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 686-785 of human SEMA3F (NP_004177.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:MCF7, U-87MG
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 686-785 of human SEMA3F (NP_004177.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KHVV TRVQ LHVL GRDA VHAA LFPP LSMS APPP PGAG PPTP PYQE LAQL LAQP EVGL IHQY CQGY WRHV PPSP REAP GAPR SPEP QDQK KPRN RRHH PPDT
Gene ID:6405
Uniprot:Q13275
Cellular Location:Secreted
Calculated MW:85kDa/88kDa
Observed MW:110kDa
Synonyms:SEMA3F, SEMA-IV, SEMA4, SEMAK
Background:This gene encodes a member of the semaphorin III family of secreted signaling proteins that are involved in axon guidance during neuronal development. The encoded protein contains an N-terminal Sema domain, an immunoglobulin loop and a C-terminal basic domain. This gene is expressed by the endothelial cells where it was found to act in an autocrine fashion to induce apoptosis, inhibit cell proliferation and survival, and function as an anti-tumorigenic agent. Alternative splicing results in multiple transcript variants encoding different isoforms.
UniProt Protein Function:SEMA3F: May play a role in cell motility and cell adhesion. Belongs to the semaphorin family.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p21.31

Molecular Function:chemorepellent activity

Biological Process: axon extension involved in axon guidance; axon guidance; nerve development

NCBI Summary:This gene encodes a member of the semaphorin III family of secreted signaling proteins that are involved in axon guidance during neuronal development. The encoded protein contains an N-terminal Sema domain, an immunoglobulin loop and a C-terminal basic domain. This gene is expressed by the endothelial cells where it was found to act in an autocrine fashion to induce apoptosis, inhibit cell proliferation and survival, and function as an anti-tumorigenic agent. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]
UniProt Code:Q13275
NCBI GenInfo Identifier:8134696
NCBI Gene ID:6405
NCBI Accession:Q13275.2
UniProt Secondary Accession:Q13275,Q13274, Q13372, Q15704, Q6GTR4, C9JQ85,
UniProt Related Accession:Q13275
Molecular Weight:88kDa
NCBI Full Name:Semaphorin-3F
NCBI Synonym Full Names:semaphorin 3F
NCBI Official Symbol:SEMA3F  
NCBI Official Synonym Symbols:SEMA4; SEMAK; SEMA-IV  
NCBI Protein Information:semaphorin-3F
UniProt Protein Name:Semaphorin-3F
UniProt Synonym Protein Names:Sema III/F; Semaphorin IV; Sema IV
Protein Family:Semaphorin
UniProt Gene Name:SEMA3F  
Anti-SEMA3F Antibody (CAB11713)Western blot analysis of extracts of various cell lines, using SEMA3F antibody (CAB11713) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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