Name: Anti-SEMA3B Antibody (CAB7004)
Brand: Cell Biology Antibodies 10
SKU: CAB7004
Availability: OutOfStock

Antibody Name:	Anti-SEMA3B Antibody
Antibody SKU:	CAB7004
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 569-748 of human SEMA3B (NP_001005914.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, HeLa, Mouse kidney, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 569-748 of human SEMA3B (NP_001005914.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SSRP ALLE HKVF GVEG SSAF LECE PRSL QARV EWTF QRAG VTAH TQVL AEER TERT ARGL LLRR LRRR DSGV YLCA AVEQ GFTQ PLRR LSLH VLSA TQAE RLAR AEEA APAA PPGP KLWY RDFL QLVE PGGG GSAN SLRM CRPQ PALQ SLPL ESRR KGRN RRTH APEP RAER GPRS ATHW
Gene ID:	7869
Uniprot:	Q13214
Cellular Location:	Endoplasmic reticulum, Secreted
Calculated MW:	83kDa
Observed MW:	83kDa

Synonyms:	SEMA3B, LUCA-1, SEMA5, SEMAA, SemA, semaV
Background:	The protein encoded by this gene belongs to the class-3 semaphorin/collapsin family, whose members function in growth cone guidance during neuronal development. This family member inhibits axonal extension and has been shown to act as a tumor suppressor by inducing apoptosis. Alternative splicing of this gene results in multiple transcript variants.

UniProt Protein Function:	SEMA3B: Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Expressed abundantly but differentially in a variety of neural and nonneural tissues. Belongs to the semaphorin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted, signal peptide; Motility/polarity/chemotaxis; Cell development/differentiation; Secreted Chromosomal Location of Human Ortholog: 3p21.3 Cellular Component: membrane; endoplasmic reticulum Molecular Function:receptor activity Biological Process: axon guidance; cell-cell signaling
NCBI Summary:	The protein encoded by this gene belongs to the class-3 semaphorin/collapsin family, whose members function in growth cone guidance during neuronal development. This family member inhibits axonal extension and has been shown to act as a tumor suppressor by inducing apoptosis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014]
UniProt Code:	Q13214
NCBI GenInfo Identifier:	8134673
NCBI Gene ID:	7869
NCBI Accession:	Q13214.1
UniProt Secondary Accession:	Q13214,Q6GU46, Q8TB71, Q8TDV7, Q93018, Q96GX0,
UniProt Related Accession:	Q13214
Molecular Weight:	749
NCBI Full Name:	Semaphorin-3B
NCBI Synonym Full Names:	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B
NCBI Official Symbol:	SEMA3B
NCBI Official Synonym Symbols:	SemA; SEMA5; SEMAA; semaV; LUCA-1
NCBI Protein Information:	semaphorin-3B; sema A(V); semaphorin A; semaphorin-V
UniProt Protein Name:	Semaphorin-3B
UniProt Synonym Protein Names:	Sema A(V); Semaphorin-V; Sema V
Protein Family:	Semaphorin
UniProt Gene Name:	SEMA3B
UniProt Entry Name:	SEM3B_HUMAN

Western blot analysis of extracts of various cell lines, using SEMA3B Antibody (CAB7004) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

ELISA Kits

Human Semaphorin 3B / SEMA3B ELISA Kit

Human SEMA3B (Semaphorin 3B) ELISA Kit (HUES01749)

Human Semaphorin-3B (SEMA3B) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)