Cell Biology Antibodies 7
Anti-SELPLG Antibody (CAB1660)
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- SKU:
- CAB1660
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-SELPLG Antibody |
| Antibody SKU: | CAB1660 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-280 of human SELPLG (NP_002997.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | DU145, Jurkat, U-937 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-280 of human SELPLG (NP_002997.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RRQA TEYE YLDY DFLP ETEP PEML RNST DTTP LTGP GTPE STTV EPAA RRST GLDA GGAV TELT TELA NMGN LSTD SAAM EIQT TQPA ATEA QTTP LAAT EAQT TRLT ATEA QTTP LAAT EAQT TPPA ATEA QTTQ PTGL EAQT TAPA AMEA QTTA PAAM EAQT TPPA AMEA QTTQ TTAM EAQT TAPE ATEA QTTQ PTAT EAQT TPLA AMEA LSTE PSAT EALS MEPT TKRG LFIP FSVS S |
| Gene ID: | 6404 |
| Uniprot: | Q14242 |
| Cellular Location: | Membrane, Single-pass type I membrane protein |
| Calculated MW: | 43kDa/44kDa |
| Observed MW: | 52kDa |
| Synonyms: | SELPLG, CD162, CLA, PSGL-1, PSGL1 |
| Background: | This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants. |
| UniProt Protein Function: | SELPLG: A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 12q24 Cellular Component: membrane; integral to plasma membrane; plasma membrane; integral to membrane Molecular Function:protein binding; receptor binding Biological Process: leukocyte tethering or rolling; cell adhesion; blood coagulation; leukocyte adhesive activation; leukocyte migration |
| NCBI Summary: | This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011] |
| UniProt Code: | Q14242 |
| NCBI GenInfo Identifier: | 2498904 |
| NCBI Gene ID: | 6404 |
| NCBI Accession: | Q14242.1 |
| UniProt Secondary Accession: | Q14242,Q12775, Q6GTW7, Q8N7J7, A8K2Y0, B4DQC3, B7Z5C7 J3KMX6, |
| UniProt Related Accession: | Q14242 |
| Molecular Weight: | 412 |
| NCBI Full Name: | P-selectin glycoprotein ligand 1 |
| NCBI Synonym Full Names: | selectin P ligand |
| NCBI Official Symbol: | SELPLG |
| NCBI Official Synonym Symbols: | CLA; CD162; PSGL1; PSGL-1 |
| NCBI Protein Information: | P-selectin glycoprotein ligand 1; cutaneous lymphocyte-associated associated antigen |
| UniProt Protein Name: | P-selectin glycoprotein ligand 1 |
| UniProt Synonym Protein Names: | Selectin P ligand; CD_antigen: CD162 |
| Protein Family: | P-selectin glycoprotein ligand |
| UniProt Gene Name: | SELPLG |
| UniProt Entry Name: | SELPL_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SELPLG antibody (CAB1660) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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