Cell Biology Antibodies 3

Anti-SELP Antibody (CAB12501)

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SKU:
CAB12501
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-SELP Antibody
Antibody SKU:CAB12501
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 220-319 of human SELP (NP_002996.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 220-319 of human SELP (NP_002996.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FSFN SQCS FHCT DGYQ VNGP SKLE CLAS GIWT NKPP QCLA AQCP PLKI PERG NMTC LHSA KAFQ HQSS CSFS CEEG FALV GPEV VQCT ASGV WTAP APVC
Gene ID:6403
Uniprot:P16109
Cellular Location:Membrane, Single-pass type I membrane protein
Calculated MW:90kDa
Observed MW:91kDa
Synonyms:SELP, CD62, CD62P, GMP140, GRMP, LECAM3, PADGEM, PSEL
Background:This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented.
UniProt Protein Function:SELP: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the selectin/LECAM family.
UniProt Protein Details:

Protein type:Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 1q22-q25

Cellular Component: nucleoplasm; extracellular space; platelet alpha granule membrane; integral to plasma membrane; cytoplasm; plasma membrane; platelet dense granule membrane; external side of plasma membrane

Molecular Function:heparin binding; protein binding; lipopolysaccharide binding; glycosphingolipid binding; glycoprotein binding; calcium-dependent protein binding; sialic acid binding; fucose binding

Biological Process: platelet activation; positive regulation of leukocyte migration; response to lipopolysaccharide; defense response to Gram-negative bacterium; heterophilic cell adhesion; positive regulation of phosphoinositide 3-kinase cascade; leukocyte adhesion; platelet degranulation; regulation of integrin activation; leukocyte tethering or rolling; blood coagulation; cell adhesion; inflammatory response; leukocyte migration

Disease: Ige Responsiveness, Atopic

NCBI Summary:This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented. [provided by RefSeq, Jul 2008]
UniProt Code:P16109
NCBI GenInfo Identifier:215274139
NCBI Gene ID:6403
NCBI Accession:P16109.3
UniProt Secondary Accession:P16109,Q5R344, Q8IVD1,
UniProt Related Accession:P16109
Molecular Weight:90,834 Da
NCBI Full Name:P-selectin
NCBI Synonym Full Names:selectin P (granule membrane protein 140kDa, antigen CD62)
NCBI Official Symbol:SELP  
NCBI Official Synonym Symbols:CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM  
NCBI Protein Information:P-selectin; GMP-140; granule membrane protein 140; granulocyte membrane protein; CD62 antigen-like family member P; platelet alpha-granule membrane protein; leukocyte-endothelial cell adhesion molecule 3; platelet activation dependent granule-external membrane protein
UniProt Protein Name:P-selectin
UniProt Synonym Protein Names:CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD_antigen: CD62P
Protein Family:P-selectin
UniProt Gene Name:SELP  
UniProt Entry Name:LYAM3_HUMAN
Anti-SELP Antibody (CAB12501)Western blot analysis of extracts of mouse kidney, using SELP Antibody (CAB12501) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-SELP Antibody (CAB12501)Immunohistochemistry of paraffin-embedded human esophagus using SELP antibody (CAB12501) (40x lens).
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