Anti-SEH1L Antibody (CAB20759)
- SKU:
- CAB20759
- Product Type:
- Antibody
- Antibody Type:
- Secondary Antibody
- Host Species:
- Rabbit
- Isotype:
- Cy3 conjugated IgG
Description
| Antibody Name: | Anti-SEH1L Antibody (CAB20759) |
| Antibody SKU: | CAB20759 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 260-360 of human SEH1L (NP_112493.2). |
| Application: | Western blotting |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | K-562, Mouse kidney, Rat kidney, Rat thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 260-360 of human SEH1L (NP_112493.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SGGP TKFE IHIV AQFD NHNS QVWR VSWN ITGT VLAS SGDD GCVR LWKA NYMD NWKC TGIL KGNG SPVN GSSQ QGTS NPSL GSTI PSLQ NSLN GSSA GRKH S |
| Cellular Location: | |
| Calculated MW: | 39kDa |
| Observed MW: | 45KDa |
| Synonyms: | Seh1, SEH1A, SEH1B, SEC13L |
| Background: | The protein encoded by this gene is part of a nuclear pore complex, Nup107-160. This protein contains WD repeats and shares 34% amino acid identity with yeast Seh1 and 30% identity with yeast Sec13. All constituents of the Nup107-160 complex, including this protein, specifically localize to kinetochores in mitosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
 | Western blot analysis of extracts of various cell lines, using SEH1L antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s. |
 | Western blot analysis of extracts of K-562 cells, using SEH1L antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s. |
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