Anti-SEH1L Antibody (CAB20759)

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SKU:
CAB20759

Description

Antibody Name:Anti-SEH1L Antibody (CAB20759)
Antibody SKU:CAB20759
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 260-360 of human SEH1L (NP_112493.2).
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:K-562, Mouse kidney, Rat kidney, Rat thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 260-360 of human SEH1L (NP_112493.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SGGP TKFE IHIV AQFD NHNS QVWR VSWN ITGT VLAS SGDD GCVR LWKA NYMD NWKC TGIL KGNG SPVN GSSQ QGTS NPSL GSTI PSLQ NSLN GSSA GRKH S
Cellular Location:
Calculated MW:39kDa
Observed MW:45KDa
Synonyms:Seh1, SEH1A, SEH1B, SEC13L
Background:The protein encoded by this gene is part of a nuclear pore complex, Nup107-160. This protein contains WD repeats and shares 34% amino acid identity with yeast Seh1 and 30% identity with yeast Sec13. All constituents of the Nup107-160 complex, including this protein, specifically localize to kinetochores in mitosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Western blot analysis of extracts of various cell lines, using SEH1L antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Western blot analysis of extracts of various cell lines, using SEH1L antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Western blot analysis of extracts of K-562 cells, using SEH1L antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Western blot analysis of extracts of K-562 cells, using SEH1L antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
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Additional Information

Product Type:
Antibody
Antibody Type:
Secondary Antibody
Host Species:
Rabbit
Isotype:
Cy3 conjugated IgG
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