Antibody Name:	Anti-SEC61B Antibody [KO Validated]
Antibody SKU:	CAB15788
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-96 of human SEC61B (NP_006799.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HeLa, HepG2, SH-SY5Y, mouse brain, mouse stomach

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-96 of human SEC61B (NP_006799.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPGP TPSG TNVG SSGR SPSK AVAA RAAG STVR QRKN ASCG TRSA GRTT SAGT GGMW RFYT EDSP GLKV GPVP VLVM SLLF IASV FMLH IWGK YTRS
Gene ID:	10952
Uniprot:	P60468
Cellular Location:	Endoplasmic reticulum membrane, Single-pass membrane protein
Calculated MW:	9kDa
Observed MW:	12kDa

Synonyms:

SEC61B

Background:

The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript.

UniProt Protein Function:	SEC61B: Necessary for protein translocation in the endoplasmic reticulum. Belongs to the SEC61-beta family.
UniProt Protein Details:	Protein type:Endoplasmic reticulum; Membrane protein, integral Chromosomal Location of Human Ortholog: 9q22.33 Cellular Component: membrane; endoplasmic reticulum; integral to membrane Molecular Function:protein binding; epidermal growth factor binding Biological Process: retrograde protein transport, ER to cytosol; SRP-dependent cotranslational protein targeting to membrane; antigen processing and presentation of peptide antigen via MHC class I; ER-associated protein catabolic process; cellular protein metabolic process; translation; protein import into nucleus, translocation; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression
NCBI Summary:	The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript. [provided by RefSeq, Jul 2008]
UniProt Code:	P60468
NCBI GenInfo Identifier:	42560366
NCBI Gene ID:	10952
NCBI Accession:	P60468.2
UniProt Related Accession:	P60468
Molecular Weight:
NCBI Full Name:	Protein transport protein Sec61 subunit beta
NCBI Synonym Full Names:	SEC61 translocon beta subunit
NCBI Official Symbol:	SEC61B
NCBI Protein Information:	protein transport protein Sec61 subunit beta
UniProt Protein Name:	Protein transport protein Sec61 subunit beta
UniProt Gene Name:	SEC61B
UniProt Entry Name:	SC61B_HUMAN

	Western blot analysis of extracts of various cell lines, using SEC61B antibody (CAB15788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 2s.
	Western blot analysis of extracts from normal (control) and SEC61B knockout (KO) 293T cells, using SEC61B antibody (CAB15788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of L929 cells using SEC61B antibody (CAB15788) at dilution of 1:100. Blue: DAPI for nuclear staining.