Name: Anti-SEC61A1 Antibody (CAB11658)
Brand: Cell Biology Antibodies 2
SKU: CAB11658
Availability: OutOfStock

Antibody Name:	Anti-SEC61A1 Antibody
Antibody SKU:	CAB11658
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 310-420 of human SEC61A1 (NP_037468.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, LO2, A-549, Mouse pancreas, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 310-420 of human SEC61A1 (NP_037468.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ARFS GNLL VSLL GTWS DTSS GGPA RAYP VGGL CYYL SPPE SFGS VLED PVHA VVYI VFML GSCA FFSK TWIE VSGS SAKD VAKQ LKEQ QMVM RGHR ETSM VHEL NRYI PTA
Gene ID:	29927
Uniprot:	P61619
Cellular Location:	Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:	38kDa/52kDa
Observed MW:	52kDa

Synonyms:

SEC61A1, HNFJ4, HSEC61, SEC61, SEC61A

Background:

The protein encoded by this gene belongs to the SECY/SEC61- alpha family. It appears to play a crucial role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum. This protein found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins. This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits.

UniProt Protein Function:	SEC61A1: Plays a crucial role in the insertion of secretory and membrane polypeptides into the ER. Required for assembly of membrane and secretory proteins. Tightly associated with membrane- bound ribosomes, either directly or through adapter proteins. Belongs to the SecY/SEC61-alpha family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Endoplasmic reticulum; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 3q21.3 Cellular Component: cytosol; endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane; membrane Molecular Function:protein binding; ribosome binding Biological Process: cell growth; endoplasmic reticulum organization and biogenesis; posttranslational protein targeting to membrane; protein targeting to ER; SRP-dependent cotranslational protein targeting to membrane Disease: Hyperuricemic Nephropathy, Familial Juvenile, 4
NCBI Summary:	The protein encoded by this gene belongs to the SECY/SEC61- alpha family. It appears to play a crucial role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum. This protein found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins. This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits. [provided by RefSeq, Jul 2008]
UniProt Code:	P61619
NCBI GenInfo Identifier:	48429109
NCBI Gene ID:	29927
NCBI Accession:	P61619.2
UniProt Secondary Accession:	P61619,P38378, P57726, Q5JPF8, Q8N0Z4, Q8N3U3, Q8NC71 Q9BU16, Q9Y2R3,
UniProt Related Accession:	P61619
Molecular Weight:	52kDa
NCBI Full Name:	Protein transport protein Sec61 subunit alpha isoform 1
NCBI Synonym Full Names:	Sec61 translocon alpha 1 subunit
NCBI Official Symbol:	SEC61A1
NCBI Official Synonym Symbols:	HNFJ4; SEC61; HSEC61; SEC61A
NCBI Protein Information:	protein transport protein Sec61 subunit alpha isoform 1
UniProt Protein Name:	Protein transport protein Sec61 subunit alpha isoform 1
Protein Family:	Protein transport protein
UniProt Gene Name:	SEC61A1

Western blot analysis of extracts of various cell lines, using SEC61A1 antibody (CAB11658) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

Anti-SEC61A1 Antibody (CAB11614)

SEC61A1 Antibody (PACO12086)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)