Cell Biology Antibodies 2

Anti-SEC61A1 Antibody (CAB11614)

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SKU:
CAB11614
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-SEC61A1 Antibody
Antibody SKU:CAB11614
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 310-420 of human SEC61A1 (NP_037468.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:A-549
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 310-420 of human SEC61A1 (NP_037468.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ARFS GNLL VSLL GTWS DTSS GGPA RAYP VGGL CYYL SPPE SFGS VLED PVHA VVYI VFML GSCA FFSK TWIE VSGS SAKD VAKQ LKEQ QMVM RGHR ETSM VHEL NRYI PTA
Gene ID:29927
Uniprot:P61619
Cellular Location:Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:38kDa/52kDa
Observed MW:52kDa
Synonyms:SEC61A1, HNFJ4, HSEC61, SEC61, SEC61A
Background:The protein encoded by this gene belongs to the SECY/SEC61- alpha family. It appears to play a crucial role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum. This protein found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins. This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits.
UniProt Protein Function:SEC61A1: Plays a crucial role in the insertion of secretory and membrane polypeptides into the ER. Required for assembly of membrane and secretory proteins. Tightly associated with membrane- bound ribosomes, either directly or through adapter proteins. Belongs to the SecY/SEC61-alpha family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Endoplasmic reticulum; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: cytosol; endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane; membrane

Molecular Function:protein binding; ribosome binding

Biological Process: cell growth; endoplasmic reticulum organization and biogenesis; posttranslational protein targeting to membrane; protein targeting to ER; SRP-dependent cotranslational protein targeting to membrane

Disease: Hyperuricemic Nephropathy, Familial Juvenile, 4

NCBI Summary:The protein encoded by this gene belongs to the SECY/SEC61- alpha family. It appears to play a crucial role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum. This protein found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins. This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits. [provided by RefSeq, Jul 2008]
UniProt Code:P61619
NCBI GenInfo Identifier:48429109
NCBI Gene ID:29927
NCBI Accession:P61619.2
UniProt Secondary Accession:P61619,P38378, P57726, Q5JPF8, Q8N0Z4, Q8N3U3, Q8NC71 Q9BU16, Q9Y2R3,
UniProt Related Accession:P61619
Molecular Weight:52kDa
NCBI Full Name:Protein transport protein Sec61 subunit alpha isoform 1
NCBI Synonym Full Names:Sec61 translocon alpha 1 subunit
NCBI Official Symbol:SEC61A1  
NCBI Official Synonym Symbols:HNFJ4; SEC61; HSEC61; SEC61A  
NCBI Protein Information:protein transport protein Sec61 subunit alpha isoform 1
UniProt Protein Name:Protein transport protein Sec61 subunit alpha isoform 1
Protein Family:Protein transport protein
UniProt Gene Name:SEC61A1  
Anti-SEC61A1 Antibody (CAB11614)Western blot analysis of extracts of A-549 cells, using SEC61A1 Antibody (CAB11614) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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