Name: Anti-SEC31A Antibody (CAB9321)
Brand: Cell Biology Antibodies 12
SKU: CAB9321
Availability: OutOfStock

Antibody Name:	Anti-SEC31A Antibody
Antibody SKU:	CAB9321
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 300-500 of human SEC31A (NP_055748.2).

Application:	WB
Recommended Dilution:	WB 1:1000 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 300-500 of human SEC31A (NP_055748.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PTNT QWCF DIQW CPRN PAVL SAAS FDGR ISVY SIMG GSTD GLRQ KQVD KLSS SFGN LDPF GTGQ PLPP LQIP QQTA QHSI VLPL KKPP KWIR RPVG ASFS FGGK LVTF ENVR MPSH QGAE QQQQ QHHV FISQ VVTE KEFL SRSD QLQQ AVQS QGFI NYCQ KKID ASQT EFEK NVWS FLKV NFED DSRG KYLE LLGY RKED L
Gene ID:	22872
Uniprot:	O94979
Cellular Location:	COPII-coated vesicle membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Peripheral membrane protein
Calculated MW:	56kDa/105-134kDa
Observed MW:	165kDa

Synonyms:

SEC31A, ABP125, ABP130, HSPC275, HSPC334, SEC31L1

Background:

The protein encoded by this gene shares similarity with the yeast Sec31 protein, and is a component of the outer layer of the coat protein complex II (COPII). The encoded protein is involved in vesicle budding from the endoplasmic reticulum (ER) and contains multiple WD repeats near the N-terminus and a proline-rich region in the C-terminal half. It associates with the protein encoded by the SEC13 homolog, nuclear pore and COPII coat complex component (SEC13), and is required for ER-Golgi transport. Monoubiquitylation of this protein by CUL3-KLHL12 was found to regulate the size of COPII coats to accommodate unusually shaped cargo. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER) (PubMed:10788476). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.
NCBI Summary:	The protein encoded by this gene shares similarity with the yeast Sec31 protein, and is a component of the outer layer of the coat protein complex II (COPII). The encoded protein is involved in vesicle budding from the endoplasmic reticulum (ER) and contains multiple WD repeats near the N-terminus and a proline-rich region in the C-terminal half. It associates with the protein encoded by the SEC13 homolog, nuclear pore and COPII coat complex component (SEC13), and is required for ER-Golgi transport. Monoubiquitylation of this protein by CUL3-KLHL12 was found to regulate the size of COPII coats to accommodate unusually shaped cargo. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
UniProt Code:	O94979
NCBI GenInfo Identifier:	1207836306
NCBI Gene ID:	22872
NCBI Accession:	OWK13809.1
UniProt Secondary Accession:	O94979,Q17RR5, Q5H9P6, Q5XG74, Q659G7, Q6ZU90, Q7LCX9 Q86TJ0, B4DIW6, B7ZKZ7, B7ZL00, H7C2W3,
UniProt Related Accession:	O94979
Molecular Weight:	133kDa
NCBI Full Name:	SEC31A, partial
NCBI Synonym Full Names:	SEC31 homolog A, COPII coat complex component
NCBI Official Symbol:	SEC31A
NCBI Official Synonym Symbols:	ABP125; ABP130; HSPC275; HSPC334; SEC31L1
NCBI Protein Information:	protein transport protein Sec31A
UniProt Protein Name:	Protein transport protein Sec31A
UniProt Synonym Protein Names:	ABP125; ABP130; SEC31-like protein 1; SEC31-related protein A; Web1-like protein
Protein Family:	Protein transport protein
UniProt Gene Name:	SEC31A

Anti-SEC31A Polyclonal Antibody (CAB9321)

Western blot analysis of extracts of various cell lines, using SEC31A antibody (CAB9321) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.

Antibodies

SEC31A Antibody (PACO12085)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)