Epigenetics & Nuclear Signaling Antibodies 1

Anti-SEC14L2 Antibody (CAB10586)

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SKU:
CAB10586
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-SEC14L2 Antibody
Antibody SKU:CAB10586
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 60-270 of human SEC14L2 (NP_001191133.1).
Application:WB IF
Recommended Dilution:WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse brain, Mouse liver, Mouse lung, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 60-270 of human SEC14L2 (NP_001191133.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GRKV ETIT IIYD CEGL GLKH LWKP AVEA YGEF LCMF EENY PETL KRLF VVKA PKLF PVAY NLIK PFLS EDTR KKIM VLGA NWKE VLLK HISP DQVP VEYG GTMT DPDG NPKC KSKI NYGG DIPR KYYV RDQV KQQY EHSV QISR GSSH QVEY EILF PGCV LRWQ FMSD GADV GFGI FLKT KMGE RQRA GEMT EVLP NQRY NSHL VPED GTL
Gene ID:23541
Uniprot:O76054
Cellular Location:Cytoplasm, Nucleus
Calculated MW:36kDa/44kDa/46kDa
Observed MW:46kDa
Synonyms:SEC14L2, C22orf6, SPF, TAP, TAP1
Background:This gene encodes a cytosolic protein which belongs to a family of lipid-binding proteins including Sec14p, alpha-tocopherol transfer protein, and cellular retinol-binding protein. The encoded protein stimulates squalene monooxygenase which is a downstream enzyme in the cholesterol biosynthetic pathway. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
UniProt Protein Function:SEC14L2: Carrier protein. Binds to some hydrophobic molecules and promotes their transfer between the different cellular sites. Binds with high affinity to alpha-tocopherol. Also binds with a weaker affinity to other tocopherols and to tocotrienols. May have a transcriptional activatory activity via its association with alpha-tocopherol. Probably recognizes and binds some squalene structure, suggesting that it may regulate cholesterol biosynthesis by increasing the transfer of squalene to a metabolic active pool in the cell. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Lipid-binding

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: cytosol; nucleus

NCBI Summary:This gene encodes a cytosolic protein which belongs to a family of lipid-binding proteins including Sec14p, alpha-tocopherol transfer protein, and cellular retinol-binding protein. The encoded protein stimulates squalene monooxygenase which is a downstream enzyme in the cholesterol biosynthetic pathway. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008]
UniProt Code:O76054
NCBI GenInfo Identifier:21542232
NCBI Gene ID:23541
NCBI Accession:O76054.1
UniProt Secondary Accession:O76054,Q53EQ2, Q6PD61, Q9ULN4, B7Z8Q1, F5H3U4,
UniProt Related Accession:O76054
Molecular Weight:46kDa
NCBI Full Name:SEC14-like protein 2
NCBI Synonym Full Names:SEC14 like lipid binding 2
NCBI Official Symbol:SEC14L2  
NCBI Official Synonym Symbols:SPF; TAP; TAP1; C22orf6  
NCBI Protein Information:SEC14-like protein 2
UniProt Protein Name:SEC14-like protein 2
UniProt Synonym Protein Names:Alpha-tocopherol-associated protein; TAP; hTAP; Squalene transfer protein; Supernatant protein factor; SPF
Protein Family:SEC14-like protein
UniProt Gene Name:SEC14L2  
Anti-SEC14L2 Antibody (CAB10586)Western blot analysis of extracts of various cell lines, using SEC14L2 antibody (CAB10586) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-SEC14L2 Antibody (CAB10586)Immunofluorescence analysis of U-2 OS cells using SEC14L2 Polyclonal Antibody (CAB10586) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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