Antibody Name:	Anti-SDHA Antibody
Antibody SKU:	CAB2594
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 385-664 of human SDHA (NP_004159.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, U-251MG, PC-3, MCF7, Mouse liver, Mouse stomach, NCI-H125

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 385-664 of human SDHA (NP_004159.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ETAM IFAG VDVT KEPI PVLP TVHY NMGG IPTN YKGQ VLRH VNGQ DQIV PGLY ACGE AACA SVHG ANRL GANS LLDL VVFG RACA LSIE ESCR PGDK VPPI KPNA GEES VMNL DKLR FADG SIRT SELR LSMQ KSMQ NHAA VFRV GSVL QEGC GKIS KLYG DLKH LKTF DRGM VWNT DLVE TLEL QNLM LCAL QTIY GAEA RKES RGAH ARED YKVR IDEY DYSK PIQG QQKK PFEE HWRK HTLS YVDV GTGK VTLE YRPV IDKT LNEA DCAT VPPA IRSY
Gene ID:	6389
Uniprot:	P31040
Cellular Location:	Matrix side, Mitochondrion inner membrane, Peripheral membrane protein
Calculated MW:	56kDa/67kDa/72kDa
Observed MW:	70kDa

Synonyms:

SDHA, CMD1GG, FP, PGL5, SDH1, SDH2, SDHF

Background:

This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	SDHA: is the catalytic subunit of succinate dehydrogenase (SDH) complex II of the mitochondrial electron transport chain. Complex II contains four subunits: the flavoprotein catalytic subunit SDHA, iron-sulfur protein SDHB, and a cytochrome b560 composed of SDHC and SDHD. Interaction with SDH5 is required for FAD attachment. Responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Defects in SDHA cause defective mitochondrial oxidative phosphorylation, giving rise to heterogeneous clinical symptoms ranging from isolated organ dysfunction to multisystem disorder. Acetylation of SDHA may control entry of the substrate into the active site, thus regulating its enzymatic activity. Acetylated SDHA may be a SIRT3 substrate SIRT3 is a mitochondrial NAD(+)-dependent deacetylase. Increased succinate levels as a consequence of SDH deficiency inhibit hypoxia inducible factor-1alpha (HIF-1alpha) prolyl hydroxylases leading to sustained HIF-1alpha expression in tumours. Defects in SDHA cause of Leigh syndrome, a severe disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
UniProt Protein Details:	Protein type:Mitochondrial; Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.3.5.1; Oxidoreductase; Tumor suppressor; Energy Metabolism - oxidative phosphorylation Chromosomal Location of Human Ortholog: 5p15 Cellular Component: mitochondrial respiratory chain complex II; mitochondrion; mitochondrial inner membrane Molecular Function:protein binding; succinate dehydrogenase (ubiquinone) activity; FAD binding; succinate dehydrogenase activity Biological Process: succinate metabolic process; cellular metabolic process; nervous system development; tricarboxylic acid cycle Disease: Mitochondrial Complex Ii Deficiency; Leigh Syndrome; Paragangliomas 5; Cardiomyopathy, Dilated, 1gg
NCBI Summary:	This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
UniProt Code:	P31040
NCBI GenInfo Identifier:	1169337
NCBI Gene ID:	6389
NCBI Accession:	P31040.2
UniProt Related Accession:	P31040
Molecular Weight:
NCBI Full Name:	Succinate dehydrogenase
NCBI Synonym Full Names:	succinate dehydrogenase complex flavoprotein subunit A
NCBI Official Symbol:	SDHA
NCBI Official Synonym Symbols:	FP; PGL5; SDH1; SDH2; SDHF; CMD1GG
NCBI Protein Information:	succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
UniProt Protein Name:	Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
UniProt Synonym Protein Names:	Flavoprotein subunit of complex II; Fp
Protein Family:	Succinate dehydrogenase
UniProt Gene Name:	SDHA
UniProt Entry Name:	SDHA_HUMAN

	Western blot analysis of extracts of various cell lines, using SDHA antibody (CAB2594) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunohistochemistry of paraffin-embedded rat heart using SDHA antibody (CAB2594) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver cancer using SDHA antibody (CAB2594) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal cancer using SDHA antibody (CAB2594) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using SDHA antibody (CAB2594). Blue: DAPI for nuclear staining.