Antibody Name:	Anti-SCYL2 Antibody
Antibody SKU:	CAB13178
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SCYL2 (NP_060458.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Mouse heart, Mouse kidney, Rat brain, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SCYL2 (NP_060458.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MESM LNKL KSTV TKVT ADVT SAVM GNPV TREF DVGR HIAS GGNG LAWK IFNG TKKS TKQE VAVF VFDK KLID KYQK FEKD QIID SLKR GVQQ LTRL RHPR LLTV QHPL EESR DCLA FCTE PVFA SLAN VLGN WENL PSPI SPDI KDYK LYDV ETKY GLLQ VSEG LSFL HSSV KMVH GNIT PENI ILNK SGAW KIMG FDFC VSST NPSE QEPK FPCK EWDP NLPS LCLP NPEY LAPE YILS VSCE TASD MYSL GTVM YAVF NKGK PIFE VNKQ DIYK SFSR QLDQ LSRL GSSS LTNI PEEV
Gene ID:	55681
Uniprot:	Q6P3W7
Cellular Location:	Cytoplasm, Cytoplasmic vesicle, Endosome membrane, Golgi apparatus, clathrin-coated vesicle, perinuclear region, trans-Golgi network membrane
Calculated MW:	103kDa
Observed MW:	104kDa

Synonyms:

SCYL2, CVAK104

Background:

The protein encoded by this gene associates with clathrin-coated complexes at the plasma membrane and with endocytic coated vesicles. The encoded protein phosphorylates the beta2 subunit of the plasma membrane adapter complex AP2 and interacts with clathrin, showing involvement in clathrin-dependent pathways between the trans-Golgi network and the endosomal system. In addition, this protein has a role in the Wnt signaling pathway by targeting frizzled 5 (Fzd5) for lysosomal degradation. Two transcript variants encoding the same protein have been found for this gene.

UniProt Protein Function:	SCYL2: Component of AP2-containing clathrin coated structures at the plasma membrane or of endocytic coated vesicles. According to PubMed:15809293, probable serine/threonine-protein kinase that phosphorylates, in vitro, the beta2-subunit of the plasma membrane adapter complex AP2 and other proteins in presence of poly-L- lysine. According to PubMed:16914521, has no detectable kinase activity in vitro. May regulate clathrin-dependent trafficking between the TGN and/or the endosomal system. Belongs to the protein kinase superfamily.
UniProt Protein Details:	Protein type:Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Other group; SCY1 family Chromosomal Location of Human Ortholog: 12q23.1 Molecular Function:protein binding; receptor binding Biological Process: endosome to lysosome transport; positive regulation of receptor internalization
NCBI Summary:	The protein encoded by this gene associates with clathrin-coated complexes at the plasma membrane and with endocytic coated vesicles. The encoded protein phosphorylates the beta2 subunit of the plasma membrane adapter complex AP2 and interacts with clathrin, showing involvement in clathrin-dependent pathways between the trans-Golgi network and the endosomal system. In addition, this protein has a role in the Wnt signaling pathway by targeting frizzled 5 (Fzd5) for lysosomal degradation. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2015]
UniProt Code:	Q6P3W7
NCBI GenInfo Identifier:	74762350
NCBI Gene ID:	55681
NCBI Accession:	Q6P3W7.1
UniProt Secondary Accession:	Q6P3W7,Q96EF4, Q96ST4, Q9H7V5, Q9NVH3, Q9P2I7, A8KAB5
UniProt Related Accession:	Q6P3W7
Molecular Weight:	103,709 Da
NCBI Full Name:	SCY1-like protein 2
NCBI Synonym Full Names:	SCY1 like pseudokinase 2
NCBI Official Symbol:	SCYL2
NCBI Official Synonym Symbols:	CVAK104
NCBI Protein Information:	SCY1-like protein 2
UniProt Protein Name:	SCY1-like protein 2
UniProt Synonym Protein Names:	Coated vesicle-associated kinase of 104 kDa
Protein Family:	SCY1-like protein
UniProt Gene Name:	SCYL2
UniProt Entry Name:	SCYL2_HUMAN

	Western blot analysis of extracts of various cell lines, using SCYL2 antibody (CAB13178) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of L929 cells using SCYL2 Rabbit pAb (CAB13178) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U2OS cells using SCYL2 Rabbit pAb (CAB13178) at dilution of 1:100. Blue: DAPI for nuclear staining.