Antibody Name:	Anti-SCO2 Antibody
Antibody SKU:	CAB7051
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human SCO2 (NP_001162582.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HL-60, SKOV3, Mouse spleen, Mouse brain, Rat spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human SCO2 (NP_001162582.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MLLL TRSP TAWH RLSQ LKPR VLPG TLGG QALH LRSW LLSR QGPA ETGG QGQP QGPG LRTR LLIT GLFG AGLG GAWL ALRA EKER LQQQ KRTE ALRQ AAVG QGDF HLLD HRGR ARCK ADFR GQWV LMYF GF
Gene ID:	9997
Uniprot:	O43819
Cellular Location:	Mitochondrion
Calculated MW:	29kDa
Observed MW:	30kDa

Synonyms:

SCO2, CEMCOX1, MYP6, SCO1L

Background:

Cytochrome c oxidase (COX) catalyzes the transfer of electrons from cytochrome c to molecular oxygen, which helps to maintain the proton gradient across the inner mitochondrial membrane that is necessary for aerobic ATP production. Human COX is a multimeric protein complex that requires several assembly factors; this gene encodes one of the COX assembly factors. The encoded protein is a metallochaperone that is involved in the biogenesis of cytochrome c oxidase subunit II. Mutations in this gene are associated with fatal infantile encephalocardiomyopathy and myopia 6.

UniProt Protein Function:	SCO2: Acts as a copper chaperone, transporting copper to the Cu(A) site on the cytochrome c oxidase subunit II (COX2). Defects in SCO2 are the cause of fatal infantile cardioencephalomyopathy with cytochrome c oxidase deficiency (FIC). This disease is characterized by hypertrophic cardiomyopathy, lactic acidosis, and gliosis. Heart and skeletal muscle show reductions in cytochrome c oxidase (COX) activity, whereas liver and fibroblasts show mild COX deficiencies. Belongs to the SCO1/2 family.
UniProt Protein Details:	Protein type:Mitochondrial Chromosomal Location of Human Ortholog: 22q13.33 Cellular Component: nucleoplasm; myofibril; mitochondrion; mitochondrial inner membrane Molecular Function:copper ion binding Biological Process: cellular copper ion homeostasis; eye development; in utero embryonic development; respiratory chain complex IV assembly; response to activity; muscle system process; copper ion transport Disease: Cardioencephalomyopathy, Fatal Infantile, Due To Cytochrome C Oxidase Deficiency 1; Myopia 6
NCBI Summary:	Cytochrome c oxidase (COX) catalyzes the transfer of electrons from cytochrome c to molecular oxygen, which helps to maintain the proton gradient across the inner mitochondrial membrane that is necessary for aerobic ATP production. Human COX is a multimeric protein complex that requires several assembly factors; this gene encodes one of the COX assembly factors. The encoded protein is a metallochaperone that is involved in the biogenesis of cytochrome c oxidase subunit II. Mutations in this gene are associated with fatal infantile encephalocardiomyopathy and myopia 6. [provided by RefSeq, Oct 2014]
UniProt Code:	O43819
NCBI GenInfo Identifier:	8134662
NCBI Gene ID:	9997
NCBI Accession:	O43819.3
UniProt Secondary Accession:	O43819,Q3T1B5, Q9UK87,
UniProt Related Accession:	O43819
Molecular Weight:	29,810 Da
NCBI Full Name:	Protein SCO2 homolog, mitochondrial
NCBI Synonym Full Names:	SCO2 cytochrome c oxidase assembly protein
NCBI Official Symbol:	SCO2
NCBI Official Synonym Symbols:	MYP6; SCO1L; CEMCOX1
NCBI Protein Information:	protein SCO2 homolog, mitochondrial; SCO cytochrome oxidase deficient homolog 2
UniProt Protein Name:	Protein SCO2 homolog, mitochondrial
Protein Family:	SCO2-like protein
UniProt Gene Name:	SCO2
UniProt Entry Name:	SCO2_HUMAN

	Western blot analysis of extracts of various cell lines, using SCO2 antibody (CAB7051) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.
	Immunohistochemistry of paraffin-embedded Human colon using SCO2 Rabbit pAb (CAB7051) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Rat ovary using SCO2 Rabbit pAb (CAB7051) at dilution of 1:100 (40x lens).