Signal Transduction Antibodies 3

Anti-SCNN1A Antibody (CAB5538)

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SKU:
CAB5538
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-SCNN1A Antibody
Antibody SKU:CAB5538
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 105-225 of human SCNN1A (NP_001029.1).
Application:WB IF
Recommended Dilution:WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse lung, Mouse intestine
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 105-225 of human SCNN1A (NP_001029.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LFGE YFSY PVSL NINL NSDK LVFP AVTI CTLN PYRY PEIK EELE ELDR ITEQ TLFD LYKY SSFT TLVA GSRS RRDL RGTL PHPL QRLR VPPP PHGA RRAR SVAS SLRD NNPQ VDWK DWKI G
Gene ID:6337
Uniprot:P37088
Cellular Location:Apical cell membrane, Cell projection, Multi-pass membrane protein, cilium
Calculated MW:28kDa/73kDa/75kDa/77kDa/78kDa/81kDa
Observed MW:76kDa
Synonyms:SCNN1A, BESC2, ENaCa, ENaCalpha, SCNEA, SCNN1
Background:Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the alpha subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
UniProt Protein Function:ENaC-alpha: Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. Probable heterotrimer containing one alpha, one beta and one gamma subunit. A delta subunit can replace the alpha subunit. Interacts with the WW domains of NEDD4, NEDD4L, WWP1 and WWP2. By aldosterone. Highly expressed in kidney and lung. Detected at intermediate levels in pancreas and liver, and at low levels in heart and placenta. Isoform 1 and isoform 2 predominate in all tissues. Expression of isoform 3, isoform 4 and isoform 5 is very low or not detectable, except in lung and heart. Activated by WNK1, WNK2, WNK3 and WNK4. Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1A subfamily. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transporter, ion channel; Membrane protein, multi-pass; Channel, sodium; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: apical plasma membrane; integral to plasma membrane; plasma membrane

Molecular Function:amiloride-sensitive sodium channel activity; protein binding; WW domain binding

Biological Process: multicellular organismal water homeostasis; sodium ion homeostasis

Disease: Bronchiectasis With Or Without Elevated Sweat Chloride 2; Pseudohypoaldosteronism, Type I, Autosomal Recessive

NCBI Summary:Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the alpha subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2009]
UniProt Code:P37088
NCBI GenInfo Identifier:585966
NCBI Gene ID:6337
NCBI Accession:P37088.1
UniProt Secondary Accession:P37088,O43271, Q6GSQ6, Q9UM64, A5X2U9, B4E2Q5, C5HTZ0
UniProt Related Accession:P37088
Molecular Weight:
NCBI Full Name:Amiloride-sensitive sodium channel subunit alpha
NCBI Synonym Full Names:sodium channel epithelial 1 alpha subunit
NCBI Official Symbol:SCNN1A  
NCBI Official Synonym Symbols:BESC2; ENaCa; SCNEA; SCNN1; ENaCalpha  
NCBI Protein Information:amiloride-sensitive sodium channel subunit alpha
UniProt Protein Name:Amiloride-sensitive sodium channel subunit alpha
UniProt Synonym Protein Names:Alpha-NaCH; Epithelial Na(+) channel subunit alpha; Alpha-ENaC; ENaCA; Nonvoltage-gated sodium channel 1 subunit alpha; SCNEA
Protein Family:Amiloride-sensitive sodium channel
UniProt Gene Name:SCNN1A  
UniProt Entry Name:SCNNA_HUMAN
Anti-SCNN1A Antibody (CAB5538)Western blot analysis of extracts of various cell lines, using SCNN1A antibody (CAB5538) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-SCNN1A Antibody (CAB5538)Immunofluorescence analysis of human lung cancer cells using SCNN1A antibody (CAB5538) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-SCNN1A Antibody (CAB5538)Immunofluorescence analysis of rat lung cells using SCNN1A antibody (CAB5538) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-SCNN1A Antibody (CAB5538)Immunofluorescence analysis of mouse kidney cells using SCNN1A antibody (CAB5538) at dilution of 1:100. Blue: DAPI for nuclear staining.
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