Cell Biology Antibodies 12
Anti-SCFD1 Antibody (CAB8835)
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- SKU:
- CAB8835
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-SCFD1 Antibody |
| Antibody SKU: | CAB8835 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 373-642 of human SCFD1 (NP_057190.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, A-431, 293T, 22Rv1, DU145, Mouse liver, Mouse kidney, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 373-642 of human SCFD1 (NP_057190.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SMLS DNTA KLTS AVSS LPEL LEKK RLID LHTN VATA VLEH IKAR KLDV YFEY EEKI MSKT TLDK SLLD IISD PDAG TPED KMRL FLIY YIST QQAP SEAD LEQY KKAL TDAG CNLN PLQY IKQW KAFT KMAS APAS YGST TTKP MGLL SRVM NTGS QFVM EGVK NLVL KQQN LPVT RILD NLME MKSN PETD DYRY FDPK MLRG NDSS VPRN KNPF QEAI VFVV GGGN YIEY QNLV DYIK GKQG KHIL YGCS ELFN ATQF IKQL SQLG QK |
| Gene ID: | 23256 |
| Uniprot: | Q8WVM8 |
| Cellular Location: | Cytoplasm, Endoplasmic reticulum membrane, Golgi apparatus, Golgi stack membrane, Peripheral membrane protein |
| Calculated MW: | 62kDa/65kDa/72kDa |
| Observed MW: | 72kDa |
| Synonyms: | SCFD1, C14orf163, RA410, SLY1, SLY1P, STXBP1L2 |
| Background: |
| UniProt Protein Function: | SCFD1: involved in vesicular transport between the endoplasmic reticulum and the Golgi. A cytoplasmic, peripheral membrane protein associated with Golgi stacks and the endoplasmic reticulum |
| UniProt Protein Details: | Protein type:Vesicle Chromosomal Location of Human Ortholog: 14q12 Cellular Component: cis-Golgi network; cytosol; Golgi transport complex; Golgi-associated vesicle; plasma membrane; SNARE complex Molecular Function:protein binding; syntaxin binding Biological Process: cell morphogenesis; COPII coating of Golgi vesicle; phagocytosis; post-Golgi vesicle-mediated transport; regulation of protein transport; response to toxin; retrograde vesicle-mediated transport, Golgi to ER |
| UniProt Code: | Q8WVM8 |
| NCBI GenInfo Identifier: | 51316882 |
| NCBI Gene ID: | 23256 |
| NCBI Accession: | Q8WVM8.4 |
| UniProt Secondary Accession: | Q8WVM8,O60754, O94990, Q7Z529, Q9BZI3, Q9UNL3, Q9Y6A8 A8K2Z5, B7Z4U7, B7Z594, |
| UniProt Related Accession: | Q8WVM8 |
| Molecular Weight: | 65,061 Da |
| NCBI Full Name: | Sec1 family domain-containing protein 1 |
| NCBI Synonym Full Names: | sec1 family domain containing 1 |
| NCBI Official Symbol: | SCFD1 |
| NCBI Official Synonym Symbols: | SLY1; RA410; SLY1P; STXBP1L2; C14orf163 |
| NCBI Protein Information: | sec1 family domain-containing protein 1 |
| UniProt Protein Name: | Sec1 family domain-containing protein 1 |
| UniProt Synonym Protein Names: | SLY1 homolog; Sly1p; Syntaxin-binding protein 1-like 2 |
| Protein Family: | Sec1 family domain-containing protein |
| UniProt Gene Name: | SCFD1 |
| UniProt Entry Name: | SCFD1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SCFD1 antibody (CAB8835) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using SCFD1 Rabbit pAb (CAB8835) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using SCFD1 Rabbit pAb (CAB8835) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat lung using SCFD1 Rabbit pAb (CAB8835) at dilution of 1:200 (40x lens). |
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