Antibody Name:	Anti-SBDS Antibody
Antibody SKU:	CAB5876
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human SBDS (NP_057122.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, SKOV3, HepG2, HL-60, Mouse brain, Mouse lung, Rat lung, Rat ovary

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human SBDS (NP_057122.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSIF TPTN QIRL TNVA VVRM KRAG KRFE IACY KNKV VGWR SGVE KDLD EVLQ THSV FVNV SKGQ VAKK EDLI SAFG TDDQ TEIC KQIL TKGE VQVS DKER HTQL EQMF RDIA TIVA DKCV NPET KRPY TVIL IERA MKDI HYSV KTNK STKQ QALE VIKQ LKEK MKIE RAHM RLRF ILPV NEGK KLKE KLKP LIKV IESE DYGQ QLEI VCLI DPGC FREI DELI KKET KGKG SLEV LNLK DVEE GDEK FE
Gene ID:	51119
Uniprot:	Q9Y3A5
Cellular Location:	Cytoplasm, Nucleus, cytoskeleton, nucleolus, nucleoplasm, spindle
Calculated MW:	28kDa
Observed MW:	34kDa

Synonyms:

SBDS, CGI-97, SDS, SWDS

Background:

This gene encodes a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The encoded protein may function in RNA metabolism. Mutations within this gene are associated with Shwachman-Bodian-Diamond syndrome. An alternative transcript has been described, but its biological nature has not been determined. This gene has a closely linked pseudogene that is distally located.

UniProt Protein Function:	SBDS: Required for the assembly of mature ribosomes and ribosome biogenesis. Together with EFTUD1, triggers the GTP- dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Required for normal levels of protein synthesis. May play a role in cellular stress resistance. May play a role in cellular response to DNA damage. May play a role in cell proliferation. Defects in SBDS are the cause of Shwachman-Diamond syndrome (SDS). SDS is an autosomal recessive disorder characterized by pancreatic exocrine insufficiency, hematologic dysfunction, and skeletal abnormalities. Belongs to the SDO1/SBDS family.
UniProt Protein Details:	Protein type:Nucleolus Chromosomal Location of Human Ortholog: 7q11.21 Cellular Component: nucleoplasm; spindle pole; cytoplasm; nucleolus; nucleus Molecular Function:rRNA binding; protein binding; microtubule binding; ribosome binding Biological Process: bone marrow development; cell proliferation; leukocyte chemotaxis; ribosomal large subunit biogenesis and assembly; inner cell mass cell proliferation; mitotic spindle stabilization; bone mineralization; rRNA processing; mature ribosome assembly Disease: Shwachman-diamond Syndrome; Aplastic Anemia
NCBI Summary:	This gene encodes a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The encoded protein may function in RNA metabolism. Mutations within this gene are associated with Shwachman-Bodian-Diamond syndrome. An alternative transcript has been described, but its biological nature has not been determined. This gene has a closely linked pseudogene that is distally located. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y3A5
NCBI GenInfo Identifier:	28380824
NCBI Gene ID:	51119
NCBI Accession:	Q9Y3A5.4
UniProt Secondary Accession:	Q9Y3A5,Q96FX0, Q9NV53, A8K0P4,
UniProt Related Accession:	Q9Y3A5
Molecular Weight:	250
NCBI Full Name:	Ribosome maturation protein SBDS
NCBI Synonym Full Names:	Shwachman-Bodian-Diamond syndrome
NCBI Official Symbol:	SBDS
NCBI Official Synonym Symbols:	SDS; SWDS; CGI-97
NCBI Protein Information:	ribosome maturation protein SBDS
UniProt Protein Name:	Ribosome maturation protein SBDS
UniProt Synonym Protein Names:	Shwachman-Bodian-Diamond syndrome protein
Protein Family:	Ribosome maturation protein
UniProt Gene Name:	SBDS
UniProt Entry Name:	SBDS_HUMAN

	Western blot analysis of extracts of various cell lines, using SBDS antibody (CAB5876) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded human gastric cancer using SBDS antibody (CAB5876) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF-7 cells using SBDS antibody (CAB5876). Blue: DAPI for nuclear staining.