Epigenetics & Nuclear Signaling Antibodies 1

Anti-SART3 Antibody (CAB12124)

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SKU:
CAB12124
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-SART3 Antibody
Antibody SKU:CAB12124
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 664-963 of human SART3 (NP_055521.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:U-87MG, A-431, HeLa, HepG2, Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 664-963 of human SART3 (NP_055521.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AGPA GKCA AVDV EPPS KQKE KAAS LKRD MPKV LHDS SKDS ITVF VSNL PYSM QEPD TKLR PLFE ACGE VVQI RPIF SNRG DFRG YCYV EFKE EKSA LQAL EMDR KSVE GRPM FVSP CVDK SKNP DFKV FRYS TSLE KHKL FISG LPFS CTKE ELEE ICKA HGTV KDLR LVTN RAGK PKGL AYVE YENE SQAS QAVM KMDG MTIK ENII KVAI SNPP QRKV PEKP ETRK APGG PMLL PQTY GARG KGRT QLSL LPRA LQRP SAAA PQAE NGPA AAPA VAAP AATE APKM SNAD FAKL FLRK
Gene ID:9733
Uniprot:Q15020
Cellular Location:Cajal body, Cytoplasm, Nucleus, Nucleus speckle, nucleoplasm
Calculated MW:13kDa/41kDa/105kDa/109kDa
Observed MW:130kDa
Synonyms:SART3, DSAP1, P100, RP11-13G14, TIP110, p110, p110(nrb)
Background:The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing.
UniProt Protein Function:SART3: a ubiquitously expressed protein in proliferating cells. A component of the 7SK snRNP complex composed of P-TEFb (composed of CDK9 and CCNT1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Transiently associates with the U6 and U4/U6 snRNPs during the spliceosome cycle, helping to regenerate functional U4/U6 snRNPs complexes. Expressed in the nucleus of all of malignant tumor cell lines tested and the majority of cancer tissues with various histologies, including squamous cell carcinomas, adenocarcinomas, melanomas and leukemias. However, this protein is undetectable in the nucleus of nonmalignant cell lines or normal tissues, except for the testis. Expressed in the cytoplasm of all proliferating cells, including normal and malignant cells, but not in most cells of normal tissues, except for the testis and the fetal liver. Regulates Tat transactivation activity through direct interaction. May be a cellular factor for HIV-1 gene expression and viral replication. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Nuclear receptor co-regulator; RNA-binding; Transcription regulation

Chromosomal Location of Human Ortholog: 12q23.3

Cellular Component: Cajal body; nucleoplasm; nucleus; U4/U6 x U5 tri-snRNP complex

Molecular Function:histone binding; protein binding; RNA binding; U4 snRNA binding; U6 snRNA binding; U6atac snRNA binding

Biological Process: assembly of spliceosomal tri-snRNP; nuclear mRNA splicing, via spliceosome; nucleosome assembly; regulation of gene expression; spliceosomal snRNP biogenesis

Disease: Porokeratosis 3, Disseminated Superficial Actinic Type

NCBI Summary:The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing. [provided by RefSeq, Jul 2008]
UniProt Code:Q15020
NCBI GenInfo Identifier:74762140
NCBI Gene ID:9733
NCBI Accession:Q15020.1
UniProt Secondary Accession:Q15020,Q2M2H0, Q58F06, Q8IUS1, Q96J95, A8K2E4, B7ZKM0
UniProt Related Accession:Q15020
Molecular Weight:110kDa
NCBI Full Name:Squamous cell carcinoma antigen recognized by T-cells 3
NCBI Synonym Full Names:squamous cell carcinoma antigen recognized by T-cells 3
NCBI Official Symbol:SART3  
NCBI Official Synonym Symbols:P100; p110; DSAP1; TIP110; p110(nrb); RP11-13G14  
NCBI Protein Information:squamous cell carcinoma antigen recognized by T-cells 3
UniProt Protein Name:Squamous cell carcinoma antigen recognized by T-cells 3
UniProt Synonym Protein Names:Tat-interacting protein of 110 kDa
Protein Family:Squamous cell carcinoma antigen
UniProt Gene Name:SART3  
Anti-SART3 Antibody (CAB12124)Western blot analysis of extracts of various cell lines, using SART3 antibody (CAB12124) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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