Antibody Name:	Anti-SARS2 Antibody
Antibody SKU:	CAB12297
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 289-518 of human SARS2 (NP_060297.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, Mouse liver, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 289-518 of human SARS2 (NP_060297.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ARFK DLNL AGTA EVGL AGYF MDHT VAFR DLPV RMVC SSTC YRAE TNTG QEPR GLYR VHHF TKVE MFGV TGPG LEQS SQLL EEFL SLQM EILT ELGL HFRV LDMP TQEL GLPA YRKF DIEA WMPG RGRF GEVT SASN CTDF QSRR LHIM FQTE AGEL QFAH TVNA TACA VPRL LIAL LESN QQKD GSVL VPPA LQSY LGTD RITA PTHV PLQY IGPN QPRK PGLP GQPA VS
Gene ID:	54938
Uniprot:	Q9NP81
Cellular Location:	Mitochondrion matrix
Calculated MW:	58kDa
Observed MW:	50kDa

Synonyms:

SARS2, SARS, SARSM, SERS, SYS, SerRS, SerRSmt

Background:

This gene encodes the mitochondrial seryl-tRNA synthethase precursor, a member of the class II tRNA synthetase family. The mature enzyme catalyzes the ligation of Serine to tRNA(Ser) and participates in the biosynthesis of selenocysteinyl-tRNA(sec) in mitochondria. The enzyme contains an N-terminal tRNA binding domain and a core catalytic domain. It functions in a homodimeric form, which is stabilized by tRNA binding. This gene is regulated by a bidirectional promoter that also controls the expression of mitochondrial ribosomal protein S12. Both genes are within the critical interval for the autosomal dominant deafness locus DFNA4 and might be linked to this disease. Multiple transcript variants encoding different isoforms have been identified for this gene.

UniProt Protein Function:	SARS2: Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec). Defects in SARS2 are the cause of hyperuricemia pulmonary hypertension renal failure and alkalosis (HUPRA). HUPRA is a multisystem disorder characterized by onset in infancy of progressive renal failure leading to electrolyte imbalances, metabolic alkalosis, pulmonary hypertension, hypotonia, and delayed development. Affected individuals are born prematurely. Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Mitochondrial; Translation; Ligase; EC 6.1.1.11 Chromosomal Location of Human Ortholog: 19q13.2 Cellular Component: cytoplasm; mitochondrial matrix; mitochondrion Molecular Function:ATP binding; serine-tRNA ligase activity Biological Process: seryl-tRNA aminoacylation; tRNA aminoacylation for protein translation Disease: Hyperuricemia, Pulmonary Hypertension, Renal Failure, And Alkalosis
NCBI Summary:	This gene encodes the mitochondrial seryl-tRNA synthethase precursor, a member of the class II tRNA synthetase family. The mature enzyme catalyzes the ligation of Serine to tRNA(Ser) and participates in the biosynthesis of selenocysteinyl-tRNA(sec) in mitochondria. The enzyme contains an N-terminal tRNA binding domain and a core catalytic domain. It functions in a homodimeric form, which is stabilized by tRNA binding. This gene is regulated by a bidirectional promoter that also controls the expression of mitochondrial ribosomal protein S12. Both genes are within the critical interval for the autosomal dominant deafness locus DFNA4 and might be linked to this disease. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Mar 2009]
UniProt Code:	Q9NP81
NCBI GenInfo Identifier:	23822219
NCBI Gene ID:	54938
NCBI Accession:	Q9NP81.1
UniProt Secondary Accession:	Q9NP81,Q9BVP3, A6NHW7, B4DE10,
UniProt Related Accession:	Q9NP81
Molecular Weight:	58,030 Da
NCBI Full Name:	Serine--tRNA ligase, mitochondrial
NCBI Synonym Full Names:	seryl-tRNA synthetase 2, mitochondrial
NCBI Official Symbol:	SARS2
NCBI Official Synonym Symbols:	SYS; SARS; SERS; SARSM; SerRS; SerRSmt; mtSerRS
NCBI Protein Information:	serine--tRNA ligase, mitochondrial
UniProt Protein Name:	Serine--tRNA ligase, mitochondrial
UniProt Synonym Protein Names:	SerRSmt; Seryl-tRNA synthetase; SerRS; Seryl-tRNA(Ser/Sec) synthetase
UniProt Gene Name:	SARS2
UniProt Entry Name:	SYSM_HUMAN

	Western blot analysis of extracts of various cell lines, using SARS2 antibody (CAB12297) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human placenta using SARS2 antibody (CAB12297) at dilution of 1:100 (40x lens).