Anti-SARS-CoV-2 Nucleoprotein Antibody (CAB21042)

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SKU:
CAB21042
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG

Description

Antibody Name:Anti-SARS-CoV-2 Nucleoprotein Antibody (CAB21042)
Antibody SKU:CAB21042
Antibody Size:50µL, 100µL
Application:Western blotting, Immunofluorescence
Reactivity:SARS-CoV-2
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-420 of coronavirus Nucleoprotein (P0DTC9).
Application:Western blotting, Immunofluorescence
Recommended Dilution:WB 1:500 - 1:2000
IF 1:50 - 1:200
Reactivity:SARS-CoV-2
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-420 of coronavirus Nucleoprotein (P0DTC9).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSDN GPQN QRNA PRIT FGGP SDST GSNQ NGER SGAR SKQR RPQG LPNN TASW FTAL TQHG KEDL KFPR GQGV PINT NSSP DDQI GYYR RATR RIRG GDGK MKDL SPRW YFYY LGTG PEAG LPYG ANKD GIIW VATE GALN TPKD HIGT RNPA NNAA IVLQ LPQG TTLP KGFY AEGS RGGS QASS RSSS RSRN SSRN STPG SSRG TSPA RMAG NGGD AALA LLLL DRLN QLES KMSG KGQQ QQGQ TVTK KSAA EASK KPRQ KRTA TKAY NVTQ AFGR RGPE QTQG NFGD QELI RQGT DYKH WPQI AQFA PSAS AFFG MSRI GMEV TPSG TWLT YTGA IKLD DKDP NFKD QVIL LNKH IDAY KTFP PTEP KKDK KKKA DETQ ALPQ RQKK QQTV TLLP AADL DDFS KQLQ QSMS SADS TQA
Cellular Location:
Calculated MW:45KDa
Observed MW:50KDa
Synonyms:
Background:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The nucleocapsid phosphoprotein is a structural protein that binds to, protects the viral RNA genome and is involved in packaging the RNA into virus particles. The N protein has been suggested as an antiviral drug target.
Immunofluorescence analysis of 293T-N and 293T cells using SARS-CoV-2 Nucleoprotein Rabbit mAb at dilution of 1:100, 1:400, 1:1600, 1:3200 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of 293T-N and 293T cells using SARS-CoV-2 Nucleoprotein Rabbit mAb at dilution of 1:100, 1:400, 1:1600, 1:3200 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of normal 293T cells and 293T transfected with Nucleoprotein Protein, using SARS-CoV-2 Nucleoprotein antibody at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 8s.Western blot analysis of extracts of normal 293T cells and 293T transfected with Nucleoprotein Protein, using SARS-CoV-2 Nucleoprotein antibody at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 8s.
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