Anti-SARS-CoV-2 3CLpro Antibody (CAB20831)

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SKU:
CAB20831
Product Type:
Antibody
Antibody Type:
Secondary Antibody
Host Species:
Goat
Isotype:
Alexa Fluor 647 conjugated IgG

Description

Antibody Name:Anti-SARS-CoV-2 3CLpro Antibody (CAB20831)
Antibody SKU:CAB20831
Antibody Size:50µL, 100µL
Application:Western blotting, Immunofluorescence
Reactivity:SARS-CoV-2
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-306 of coronavirus 3CLpro (YP_009725295.1).
Application:Western blotting, Immunofluorescence
Recommended Dilution:WB 1:500 - 1:2000
IF 1:50 - 1:200
Reactivity:SARS-CoV-2
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-306 of coronavirus 3CLpro (YP_009725295.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MESL VPGF NEKT HVQL SLPV LQVR DVLV RGFG DSVE EVLS EARQ HLKD GTCG LVEV EKGV LPQL EQPY VFIK RSDA RTAP HGHV MVEL VAEL EGIQ YGRS GETL GVLV PHVG EIPV AYRK VLLR KNGN KGAG GHSY GADL KSFD LGDE LGTD PYED FQEN WNTK HSSG VTRE LMRE LNGG AYTR YVDN NFCG PDGY PLEC IKDL LARA GKAS CTLS EQLD FIDT KRGV YCCR EHEH EIAW YTER SEKS YELQ TPFE IKLA KKFD TFNG ECPN FVFP LNSI IKTI QPRV EKKK LDGF MGRI RSVY PV
Cellular Location:
Calculated MW:
Observed MW:30-35KDa
Synonyms:
Background:Spike protein S1: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein. Binding to host NRP1 and NRP2 via C-terminal polybasic sequence enhances virion entry into host cell. This interaction may explain virus tropism of human olfactory epithelium cells, which express high level of NRP1 and NRP2 but low level of ACE2. The stalk domain of S contains three hinges, giving the head unexpected orientational freedom. Uses human TMPRSS2 for priming in human lung cells which is an essential step for viral entry. Can be alternatively processed by host furin. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.
Immunofluorescence analysis of 293T cells transfected with SARS-Cov-2 3CLpro and untreated 293T cells using SARS-CoV-2 3CLpro Rabbit mAb at dilution of 1:100/1:400/1:800(40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of 293T cells transfected with SARS-Cov-2 3CLpro and untreated 293T cells using SARS-CoV-2 3CLpro Rabbit mAb at dilution of 1:100/1:400/1:800(40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of 293T cells, using SARS-CoV-2 3CLpro antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.Western blot analysis of extracts of 293T cells, using SARS-CoV-2 3CLpro antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
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