Metabolism Antibodies 2
Anti-SARS Antibody (CAB6733)
- SKU:
- CAB6733
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-SARS Antibody |
| Antibody SKU: | CAB6733 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SARS (NP_006504.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, HepG2, HeLa, U-251MG, 293T, Mouse kidney, Mouse brain, Rat kidney, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SARS (NP_006504.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MVLD LDLF RVDK GGDP ALIR ETQE KRFK DPGL VDQL VKAD SEWR RCRF RADN LNKL KNLC SKTI GEKM KKKE PVGD DESV PENV LSFD DLTA DALA NLKV SQIK KVRL LIDE AILK CDAE RIKL EAER FENL REIG NLLH PSVP ISND EDVD NKVE RIWG DCTV RKKY SHVD LVVM VDGF EGEK GAVV AGSR GYFL KGVL VFLE QALI QYAL RTLG SRGY IPIY TPFF MRKE VMQE VAQL SQFD EELY KVIG KGSE KSDD NSYD EKYL IATS EQPI AALH RDEW LRPE DLPI KYAG LSTC |
| Gene ID: | 6301 |
| Uniprot: | P49591 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 58kDa |
| Observed MW: | 59kDa |
| Synonyms: | SARS, SERRS, SERS |
| Background: | This gene belongs to the class II amino-acyl tRNA family. The encoded enzyme catalyzes the transfer of L-serine to tRNA (Ser) and is related to bacterial and yeast counterparts. Multiple alternatively spliced transcript variants have been described but the biological validity of all variants is unknown. |
| UniProt Protein Function: | SARS: Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec). Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily. |
| UniProt Protein Details: | Protein type:Translation; EC 6.1.1.11; RNA processing; Ligase Chromosomal Location of Human Ortholog: 1p13.3 Cellular Component: cytoplasm; cytosol Molecular Function:RNA binding; serine-tRNA ligase activity Biological Process: selenocysteine metabolic process; seryl-tRNA aminoacylation; translation; tRNA aminoacylation for protein translation; tRNA processing |
| NCBI Summary: | This gene belongs to the class II amino-acyl tRNA family. The encoded enzyme catalyzes the transfer of L-serine to tRNA (Ser) and is related to bacterial and yeast counterparts. Multiple alternatively spliced transcript variants have been described but the biological validity of all variants is unknown. [provided by RefSeq, Jul 2010] |
| UniProt Code: | P49591 |
| NCBI GenInfo Identifier: | 19860217 |
| NCBI Gene ID: | 6301 |
| NCBI Accession: | P49591.3 |
| UniProt Secondary Accession: | P49591,Q5T5C8, Q9NSE3, B2R6Y9, |
| UniProt Related Accession: | P49591 |
| Molecular Weight: | 58,777 Da |
| NCBI Full Name: | Serine--tRNA ligase, cytoplasmic |
| NCBI Synonym Full Names: | seryl-tRNA synthetase |
| NCBI Official Symbol: | SARS |
| NCBI Official Synonym Symbols: | SERS; SERRS |
| NCBI Protein Information: | serine--tRNA ligase, cytoplasmic |
| UniProt Protein Name: | Serine--tRNA ligase, cytoplasmic |
| UniProt Synonym Protein Names: | Seryl-tRNA synthetase; SerRS; Seryl-tRNA(Ser/Sec) synthetase |
| Protein Family: | Serine--tRNA ligase |
| UniProt Gene Name: | SARS |
| UniProt Entry Name: | SYSC_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SARS antibody (CAB6733) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat ovary using SARS Rabbit pAb (CAB6733) at dilution of 1:50 (40x lens). |
 | Immunofluorescence analysis of MCF7 cells using SARS antibody (CAB6733). Blue: DAPI for nuclear staining. |
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