Description
Antibody Name: | Anti-SAMM50 Antibody (CAB9355) |
Antibody SKU: | CAB9355 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting, Immunohistochemistry |
Reactivity: | Human, Mouse, Rat |
Host Species: | Rabbit |
Immunogen: | A synthesized peptide derived from human SAMM50. |
Application: | Western blotting, Immunohistochemistry |
Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
Reactivity: | Human, Mouse, Rat |
Positive Samples: | 293T, HepG2, RD, Mouse heart, Mouse skeletal muscle, Rat heart, Rat pancreas |
Immunogen: | A synthesized peptide derived from human SAMM50. |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | Email for sequence |
Cellular Location: | Cytoplasm, Mitochondrion, Mitochondrion outer membrane, Multi-pass membrane protein |
Calculated MW: | 52kDa |
Observed MW: | 52KDa |
Synonyms: | SAMM50, CGI-51, OMP85, SAM50, TOB55, TRG-3, YNL026W, SAMM50 sorting and assembly machinery component |
Background: | This gene encodes a component of the Sorting and Assembly Machinery (SAM) of the mitochondrial outer membrane. The Sam complex functions in the assembly of beta-barrel proteins into the outer mitochondrial membrane. |
Immunohistochemistry of paraffin-embedded mouse stomach using SAMM50 Rabbit mAb at dilution of 1:250 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Immunohistochemistry of paraffin-embedded rat kidney using SAMM50 Rabbit mAb at dilution of 1:250 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Immunohistochemistry of paraffin-embedded mouse kidney using SAMM50 Rabbit mAb at dilution of 1:250 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Immunohistochemistry of paraffin-embedded human lung cancer using SAMM50 Rabbit mAb at dilution of 1:250 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Immunohistochemistry of paraffin-embedded human liver cancer using SAMM50 Rabbit mAb at dilution of 1:250 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Immunohistochemistry of paraffin-embedded human colon carcinoma using SAMM50 Rabbit mAb at dilution of 1:250 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Western blot analysis of extracts of various cell lines, using at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |