Cell Biology Antibodies 8
Anti-SAMM50 Antibody (CAB3401)
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- SKU:
- CAB3401
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-SAMM50 Antibody |
| Antibody SKU: | CAB3401 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human SAMM50 (NP_056195.3). |
| Application: | WB IHC IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, A-431, LO2, HL-60, HT-1080, Mouse eye, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human SAMM50 (NP_056195.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGTV HARS LEPL PSSG PDFG GLGE EAEF VEVE PEAK QEIL ENKD VVVQ HVHF DGLG RTKD DIII CEIG DVFK AKNL IEVM RKSH EARE KLLR LGIF RQVD VLID TCQG DDAL PNGL DVTF EVTE LRRL TGSY NTMV GNNE GSMV LGLK LPNL LGRA EKVT FQFS YGTK ETSY GLSF FKPR PGNF ERNF SVNL YKVT GQFP WSSL RETD RGMS AEYS FPIW |
| Gene ID: | 25813 |
| Uniprot: | Q9Y512 |
| Cellular Location: | Cytoplasm, Mitochondrion, Mitochondrion outer membrane, Multi-pass membrane protein |
| Calculated MW: | 51kDa |
| Observed MW: | 55kDa |
| Synonyms: | SAMM50, CGI-51, OMP85, SAM50, TOB55, TRG-3, YNL026W |
| Background: | This gene encodes a component of the Sorting and Assembly Machinery (SAM) of the mitochondrial outer membrane. The Sam complex functions in the assembly of beta-barrel proteins into the outer mitochondrial membrane. |
| UniProt Protein Function: | SAMM50: May be required for the assembly pathway of mitochondrial outer membrane proteins. Belongs to the SAM50/omp85 family. |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; Mitochondrial Chromosomal Location of Human Ortholog: 22q13.31 Cellular Component: mitochondrial outer membrane; mitochondrion; mitochondrial inner membrane; integral to membrane Molecular Function:protein binding Biological Process: cellular protein metabolic process; protein targeting to mitochondrion; protein import into mitochondrial outer membrane |
| NCBI Summary: | This gene encodes a component of the Sorting and Assembly Machinery (SAM) of the mitochondrial outer membrane. The Sam complex functions in the assembly of beta-barrel proteins into the outer mitochondrial membrane.[provided by RefSeq, Jun 2011] |
| UniProt Code: | Q9Y512 |
| NCBI GenInfo Identifier: | 118572715 |
| NCBI Gene ID: | 25813 |
| NCBI Accession: | Q9Y512.3 |
| UniProt Related Accession: | Q9Y512 |
| Molecular Weight: | ~ 52kDa |
| NCBI Full Name: | Sorting and assembly machinery component 50 homolog |
| NCBI Synonym Full Names: | SAMM50 sorting and assembly machinery component |
| NCBI Official Symbol: | SAMM50 |
| NCBI Official Synonym Symbols: | OMP85; SAM50; TOB55; TRG-3; CGI-51; YNL026W |
| NCBI Protein Information: | sorting and assembly machinery component 50 homolog |
| UniProt Protein Name: | Sorting and assembly machinery component 50 homolog |
| UniProt Synonym Protein Names: | Transformation-related gene 3 protein; TRG-3 |
| Protein Family: | Sorting and assembly machinery component |
| UniProt Gene Name: | SAMM50 |
| UniProt Entry Name: | SAM50_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SAMM50 antibody (CAB3401) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat heart using SAMM50 Rabbit pAb (CAB3401) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver cancer using SAMM50 Rabbit pAb (CAB3401) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse heart using SAMM50 Rabbit pAb (CAB3401) at dilution of 1:200 (40x lens). |
 | Immunoprecipitation analysis of 600ug extracts of Mouse heart cells using 3ug SAMM50 antibody (CAB3401). Western blot was performed from the immunoprecipitate using SAMM50 antibody (CAB3401) at a dilition of 1:1000. |
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