Neuroscience

Anti-SAG Antibody (CAB13045)

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SKU:
CAB13045
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Neuroscience

Description

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Antibody Name:Anti-SAG Antibody
Antibody SKU:CAB13045
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-405 of human SAG (NP_000532.2).
Application:WB IHC
Recommended Dilution:WB 1:1000 - 1:2000 IHC 1:50 - 1:200
Reactivity:Mouse, Rat
Positive Samples:Mouse eye, Rat eye
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-405 of human SAG (NP_000532.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAS GKTS KSEP NHVI FKKI SRDK SVTI YLGN RDYI DHVS QVQP VDGV VLVD PDLV KGKK VYVT LTCA FRYG QEDI DVIG LTFR RDLY FSRV QVYP PVGA ASTP TKLQ ESLL KKLG SNTY PFLL TFPD YLPC SVML QPAP QDSG KSCG VDFE VKAF ATDS TDAE EDKI PKKS SVRL LIRK VQHA PLEM GPQP RAEA AWQF FMSD KPLH LAVS LNKE IYFH GEPI PVTV TVTN NTEK TVKK IKAF VEQV ANVV LYSS DYYV KPVA MEEA QEKV PPNS TLTK TLTL LPLL ANNR ERRG IALD GKIK HEDT NLAS STII KEGI DRTV LGIL VSYQ IKVK LTVS GFLG ELTS SEVA TEVP FRLM HPQP EDPA KESY QDAN LVFE EFAR HNLK DAGE AEEG KRDK NDVD E
Gene ID:6295
Uniprot:P10523
Cellular Location:
Calculated MW:45kDa
Observed MW:48kDa
Synonyms:SAG, RP47, S-AG
Background:Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. S-arrestin, also known as S-antigen, is a major soluble photoreceptor protein that is involved in desensitization of the photoactivated transduction cascade. It is expressed in the retina and the pineal gland and inhibits coupling of rhodopsin to transducin in vitro. Additionally, S-arrestin is highly antigenic, and is capable of inducing experimental autoimmune uveoretinitis. Mutations in this gene have been associated with Oguchi disease, a rare autosomal recessive form of night blindness.
UniProt Protein Function:SAG: Arrestin is one of the major proteins of the ros (retinal rod outer segments); it binds to photoactivated- phosphorylated rhodopsin, thereby apparently preventing the transducin-mediated activation of phosphodiesterase. Retina and pineal gland. Belongs to the arrestin family.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; G protein regulator, misc.

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: cytosol

Molecular Function:protein phosphatase inhibitor activity

Biological Process: cell surface receptor linked signal transduction; regulation of rhodopsin mediated signaling; rhodopsin mediated signaling

Disease: Oguchi Disease 1; Retinitis Pigmentosa 47

NCBI Summary:Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. S-arrestin, also known as S-antigen, is a major soluble photoreceptor protein that is involved in desensitization of the photoactivated transduction cascade. It is expressed in the retina and the pineal gland and inhibits coupling of rhodopsin to transducin in vitro. Additionally, S-arrestin is highly antigenic, and is capable of inducing experimental autoimmune uveoretinitis. Mutations in this gene have been associated with Oguchi disease, a rare autosomal recessive form of night blindness. [provided by RefSeq, Jul 2008]
UniProt Code:P10523
NCBI GenInfo Identifier:109940055
NCBI Gene ID:6295
NCBI Accession:P10523.3
UniProt Secondary Accession:P10523,Q53SV3, Q99858, A0FDN6,
UniProt Related Accession:P10523
Molecular Weight:45,120 Da
NCBI Full Name:S-arrestin
NCBI Synonym Full Names:S-antigen visual arrestin
NCBI Official Symbol:SAG  
NCBI Official Synonym Symbols:RP47; S-AG  
NCBI Protein Information:S-arrestin
UniProt Protein Name:S-arrestin
UniProt Synonym Protein Names:48 kDa protein; Retinal S-antigen; S-AG; Rod photoreceptor arrestin
Protein Family:S-arrestin
UniProt Gene Name:SAG  
UniProt Entry Name:ARRS_HUMAN
Anti-SAG Antibody (CAB13045)Western blot analysis of extracts of various cell lines, using SAG antibody (CAB13045) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-SAG Antibody (CAB13045)Immunohistochemistry of paraffin-embedded mouse retina using SAG antibody (CAB13045) at dilution of 1:200 (40x lens).
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