Signal Transduction Antibodies 3
Anti-SAE1 Antibody (CAB9960)
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- SKU:
- CAB9960
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-SAE1 Antibody |
| Antibody SKU: | CAB9960 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-346 of human SAE1 (NP_005491.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, B cells, K-562, HT-29, LO2, NIH/3T3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-346 of human SAE1 (NP_005491.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MVEK EEAG GGIS EEEA AQYD RQIR LWGL EAQK RLRA SRVL LVGL KGLG AEIA KNLI LAGV KGLT MLDH EQVT PEDP GAQF LIRT GSVG RNRA EASL ERAQ NLNP MVDV KVDT EDIE KKPE SFFT QFDA VCLT CCSR DVIV KVDQ ICHK NSIK FFTG DVFG YHGY TFAN LGEH EFVE EKTK VAKV SQGV EDGP DTKR AKLD SSET TMVK KKVV FCPV KEAL EVDW SSEK AKAA LKRT TSDY FLLQ VLLK FRTD KGRD PSSD TYEE DSEL LLQI RNDV LDSL GISP DLLP EDFV RYCF SEMA PVCA VVGG ILAQ EIVK ALSQ RDPP HNNF FFFD GMKG NGIV ECLG PK |
| Gene ID: | 10055 |
| Uniprot: | Q9UBE0 |
| Cellular Location: | Nucleus |
| Calculated MW: | 29kDa/33kDa/38kDa |
| Observed MW: | 38kDa |
| Synonyms: | SAE1, AOS1, HSPC140, SUA1, UBLE1A |
| Background: |
| UniProt Protein Function: | UBLE1A: The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. Belongs to the ubiquitin-activating E1 family. |
| UniProt Protein Details: | Protein type:Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.19 Chromosomal Location of Human Ortholog: 19q13.32 Cellular Component: cytosol; nucleoplasm; nucleus Molecular Function:ATP-dependent protein binding; enzyme activator activity; protein binding; protein C-terminus binding; protein heterodimerization activity; SUMO activating enzyme activity; ubiquitin activating enzyme activity Biological Process: protein sumoylation; protein ubiquitination; regulation of mitotic cell cycle |
| NCBI Summary: | Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010] |
| UniProt Code: | Q9UBE0 |
| NCBI GenInfo Identifier: | 42559897 |
| NCBI Gene ID: | 10055 |
| NCBI Accession: | Q9UBE0.1 |
| UniProt Secondary Accession: | Q9UBE0,O95717, Q9P020, B2RDP5, B3KMQ2, F5GXX7, G3XAK6 |
| UniProt Related Accession: | Q9UBE0 |
| Molecular Weight: | 33,063 Da |
| NCBI Full Name: | SUMO-activating enzyme subunit 1 |
| NCBI Synonym Full Names: | SUMO1 activating enzyme subunit 1 |
| NCBI Official Symbol: | SAE1 |
| NCBI Official Synonym Symbols: | AOS1; SUA1; UBLE1A; HSPC140 |
| NCBI Protein Information: | SUMO-activating enzyme subunit 1 |
| UniProt Protein Name: | SUMO-activating enzyme subunit 1 |
| UniProt Synonym Protein Names: | Ubiquitin-like 1-activating enzyme E1A |
| Protein Family: | SUMO-activating enzyme |
| UniProt Gene Name: | SAE1 |
| UniProt Entry Name: | SAE1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SAE1 antibody (CAB9960) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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