Cardiovascular Antibodies

Anti-S1PR1 Antibody (CAB12935)

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SKU:
CAB12935
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cardiovascular

Description

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Antibody Name:Anti-S1PR1 Antibody
Antibody SKU:CAB12935
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 303-382 of human S1PR1 (NP_001391.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:NIH/3T3, SH-SY5Y, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 303-382 of human S1PR1 (NP_001391.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:NSGT NPII YTLT NKEM RRAF IRIM SCCK CPSG DSAG KFKR PIIA GMEF SRSK SDNS SHPQ KDEG DNPE TIMS SGNV NSSS
Gene ID:1901
Uniprot:P21453
Cellular Location:Cell membrane, Endosome, Membrane raft, Multi-pass membrane protein
Calculated MW:42kDa
Observed MW:43kDa
Synonyms:S1PR1, CD363, CHEDG1, D1S3362, ECGF1, EDG-1, EDG1, S1P1
Background:The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:EDG-1: a G protein-coupled receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Highly expressed in endothelial cells and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin. Suggested to be involved in the processes that regulate the migration/differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. S1P-induced endothelial cell migration requires AKT1-mediated phosphorylation of the third intracellular loop. Seems to be coupled to the G(i) subclass of heteromeric G proteins.
UniProt Protein Details:

Protein type:GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 1p21.2

Cellular Component: intracellular membrane-bound organelle; intrinsic to plasma membrane; nucleoplasm; plasma membrane

Molecular Function:G-protein coupled receptor activity; G-protein-coupled receptor binding

Biological Process: actin cytoskeleton reorganization; blood vessel maturation; cell adhesion; cell migration; chemotaxis; G-protein coupled receptor protein signaling pathway; lamellipodium biogenesis; regulation of bone mineralization; regulation of bone resorption

NCBI Summary:The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
UniProt Code:P21453
NCBI GenInfo Identifier:205371820
NCBI Gene ID:1901
NCBI Accession:P21453.2
UniProt Secondary Accession:P21453,Q9BYY4, Q9NYN8, D3DT66,
UniProt Related Accession:P21453
Molecular Weight:
NCBI Full Name:Sphingosine 1-phosphate receptor 1
NCBI Synonym Full Names:sphingosine-1-phosphate receptor 1
NCBI Official Symbol:S1PR1  
NCBI Official Synonym Symbols:EDG1; S1P1; CD363; ECGF1; EDG-1; CHEDG1; D1S3362  
NCBI Protein Information:sphingosine 1-phosphate receptor 1
UniProt Protein Name:Sphingosine 1-phosphate receptor 1
UniProt Synonym Protein Names:Endothelial differentiation G-protein coupled receptor 1; Sphingosine 1-phosphate receptor Edg-1; S1P receptor Edg-1; CD_antigen: CD363
Protein Family:Sphingosine 1-phosphate receptor
UniProt Gene Name:S1PR1  
Anti-S1PR1 Antibody (CAB12935)Western blot analysis of extracts of various cell lines, using S1PR1 antibody (CAB12935) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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