KO Validated Antibodies 1

Anti-S100A6 Antibody (CAB4104)[KO Validated]

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SKU:
CAB4104
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-S100A6 Antibody [KO Validated]
Antibody SKU:CAB4104
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human S100A6 (NP_055439.1).
Application:WB IHC IF IP
Recommended Dilution:WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:20 - 1:50
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, NIH/3T3, HT-29, U-251MG, Mouse lung, Mouse skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human S100A6 (NP_055439.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MACP LDQA IGLL VAIF HKYS GREG DKHT LSKK ELKE LIQK ELTI GSKL QDAE IARL MEDL DRNK DQEV NFQE YVTF LGAL ALIY NEAL KG
Gene ID:6277
Uniprot:P06703
Cellular Location:Cell membrane, Cytoplasm, Cytoplasmic side, Nucleus envelope, Peripheral membrane protein
Calculated MW:10kDa
Observed MW:10kDa
Synonyms:S100A6, 2A9, 5B10, CABP, CACY, PRA
Background:The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma.
UniProt Protein Function:S100A6: May function as calcium sensor and contribute to cellular calcium signaling (Potential). May function by interacting with other proteins and indirectly play a role in the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative. Belongs to the S-100 family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoplasm; cytosol; extrinsic to internal side of plasma membrane; nuclear envelope; nucleus; perinuclear region of cytoplasm; ruffle

Molecular Function:calcium ion binding; calcium-dependent protein binding; protein binding; protein homodimerization activity; tropomyosin binding

Biological Process: signal transduction

NCBI Summary:The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma. [provided by RefSeq, Jul 2008]
UniProt Code:P06703
NCBI GenInfo Identifier:116509
NCBI Gene ID:6277
NCBI Accession:P06703.1
UniProt Secondary Accession:P06703,Q5RHS4, D3DV39,
UniProt Related Accession:P06703
Molecular Weight:10,180 Da
NCBI Full Name:Protein S100-A6
NCBI Synonym Full Names:S100 calcium binding protein A6
NCBI Official Symbol:S100A6  
NCBI Official Synonym Symbols:2A9; PRA; 5B10; CABP; CACY  
NCBI Protein Information:protein S100-A6
UniProt Protein Name:Protein S100-A6
UniProt Synonym Protein Names:Calcyclin; Growth factor-inducible protein 2A9; MLN 4; Prolactin receptor-associated protein; PRA; S100 calcium-binding protein A6
Protein Family:Protein
UniProt Gene Name:S100A6  
UniProt Entry Name:S10A6_HUMAN
Anti-S100A6 Antibody [KO Validated] (CAB4104)Western blot analysis of extracts of various cell lines, using S100A6 antibody (CAB4104) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-S100A6 Antibody [KO Validated] (CAB4104)Western blot analysis of extracts from normal (control) and S100A6 knockout (KO) HeLa cells, using S100A6 antibody (CAB4104) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-S100A6 Antibody [KO Validated] (CAB4104)Immunohistochemistry of paraffin-embedded rat cerebellum using [KO Validated] S100A6 Rabbit pAb (CAB4104) at dilution of 1:100 (40x lens).
Anti-S100A6 Antibody [KO Validated] (CAB4104)Immunoprecipitation analysis of 200ug extracts of U-251MG cells using 3ug S100A6 antibody (CAB4104). Western blot was performed from the immunoprecipitate using S100A6 antibody (CAB4104) at a dilition of 1:1000.
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