Antibody Name:	Anti-S100A5 Antibody
Antibody SKU:	CAB14779
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human S100A5 (NP_002953.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human
Positive Samples:	U-251MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human S100A5 (NP_002953.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	METP LEKA LTTM VTTF HKYS GREG SKLT LSRK ELKE LIKK ELCL GEMK ESSI DDLM KSLD KNSD QEID FKEY SVFL TMLC MAYN DFFL EDNK
Gene ID:	6276
Uniprot:	P33763
Cellular Location:
Calculated MW:	10kDa/12kDa
Observed MW:	11kDa

Synonyms:

S100A5, S100D

Background:

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein has a Ca2+ affinity 20- to 100-fold higher than the other S100 proteins studied under identical conditions. This protein also binds Zn2+ and Cu2+, and Cu2+ strongly which impairs the binding of Ca2+. This protein is expressed in very restricted regions of the adult brain.

UniProt Protein Function:	S100A5: Binds calcium, zinc and copper. One subunit can simultaneously bind 2 calcium ions or 2 copper ions plus 1 zinc ion. Calcium and copper ions compete for the same binding sites. Belongs to the S-100 family.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 1q21 Cellular Component: cell soma; nucleus Molecular Function:protein homodimerization activity; copper ion binding; zinc ion binding; calcium ion binding
NCBI Summary:	The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein has a Ca2+ affinity 20- to 100-fold higher than the other S100 proteins studied under identical conditions. This protein also binds Zn2+ and Cu2+, and Cu2+ strongly which impairs the binding of Ca2+. This protein is expressed in very restricted regions of the adult brain. [provided by RefSeq, Jul 2008]
UniProt Code:	P33763
NCBI GenInfo Identifier:	62739837
NCBI Gene ID:	6276
NCBI Accession:	AAH93957.1
UniProt Secondary Accession:	P33763,Q5RHS3,
UniProt Related Accession:	P33763
Molecular Weight:	10,744 Da
NCBI Full Name:	S100 calcium binding protein A5
NCBI Synonym Full Names:	S100 calcium binding protein A5
NCBI Official Symbol:	S100A5
NCBI Official Synonym Symbols:	S100D
NCBI Protein Information:	protein S100-A5; S100 calcium-binding protein A5
UniProt Protein Name:	Protein S100-A5
UniProt Synonym Protein Names:	Protein S-100D; S100 calcium-binding protein A5
Protein Family:	Protein
UniProt Gene Name:	S100A5
UniProt Entry Name:	S10A5_HUMAN

	Western blot analysis of extracts of U-251MG cells, using S100A5 Rabbit pAb (CAB14779) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.
	Immunohistochemistry of paraffin-embedded human thyroid cancer using S100A5 antibody (CAB14779) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using S100A5 antibody (CAB14779) at dilution of 1:100 (40x lens).