Immunology Antibodies 3
Anti-RXRAlpha Antibody (CAB19105)
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- SKU:
- CAB19105
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-RXRAlpha Antibody |
| Antibody SKU: | CAB19105 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthesized peptide derived from human RXRAlpha |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, HepG2, BxPC-3, Mouse lung, Rat lung, Rat liver |
| Immunogen: | A synthesized peptide derived from human RXRAlpha |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 6256 |
| Uniprot: | P19793 |
| Cellular Location: | Nucleus |
| Calculated MW: | 51kDa |
| Observed MW: | 60kDa |
 | Western blot analysis of extracts of various cell lines, using RXRα antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min. |
 | Western blot analysis of extracts of various cell lines, using RXRα antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s. |
 | Immunofluorescence analysis of HeLa cells using RXRα Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug RXRα antibody at a dilition of 1:1000. |
 | Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using RXRα antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
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