Name: Anti-RXFP1 Antibody (CAB7127)
Brand: ChIP Antibodies
SKU: CAB7127
Availability: OutOfStock

Antibody Name:	Anti-RXFP1 Antibody
Antibody SKU:	CAB7127
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 673-757 of human RXFP1 (NP_067647.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HeLa, Mouse brain, Rat brain, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 673-757 of human RXFP1 (NP_067647.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NSAL NPIL YTLT TRPF KEMI HRFW YNYR QRKS MDSK GQKT YAPS FIWV EMWP LQEM PPEL MKPD LFTY PCEM SLIS QSTR LNSY S
Gene ID:	59350
Uniprot:	Q9HBX9
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	21kDa/75kDa/81kDa/83kDa/86kDa
Observed MW:	87kDa

Synonyms:

RXFP1, LGR7, RXFPR1

Background:

This gene encodes a member of the leucine-rich repeat-containing subgroup of the G protein-coupled 7-transmembrane receptor superfamily. The encoded protein plays a critical role in sperm motility, pregnancy and parturition as a receptor for the protein hormone relaxin. Decreased expression of this gene may play a role in endometriosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	RXFP1: Receptor for relaxins. The activity of this receptor is mediated by G proteins leading to stimulation of adenylate cyclase and an increase of cAMP. Binding of the ligand may also activate a tyrosine kinase pathway that inhibits the activity of a phosphodiesterase that degrades cAMP. Belongs to the G-protein coupled receptor 1 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 4q32.1 Cellular Component: plasma membrane; integral to membrane Molecular Function:G-protein coupled receptor activity; protein binding; metal ion binding Biological Process: G-protein coupled receptor protein signaling pathway
NCBI Summary:	This gene encodes a member of the leucine-rich repeat-containing subgroup of the G protein-coupled 7-transmembrane receptor superfamily. The encoded protein plays a critical role in sperm motility, pregnancy and parturition as a receptor for the protein hormone relaxin. Decreased expression of this gene may play a role in endometriosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
UniProt Code:	Q9HBX9
NCBI GenInfo Identifier:	166209887
NCBI Gene ID:	59350
NCBI Accession:	Q9HBX9.2
UniProt Secondary Accession:	Q9HBX9,Q2M215, Q3KU24, Q3KU25, Q3KU26, B4DHD1, B4DTV2
UniProt Related Accession:	Q9HBX9
Molecular Weight:	757
NCBI Full Name:	Relaxin receptor 1
NCBI Synonym Full Names:	relaxin/insulin-like family peptide receptor 1
NCBI Official Symbol:	RXFP1
NCBI Official Synonym Symbols:	LGR7; RXFPR1
NCBI Protein Information:	relaxin receptor 1; leucine-rich repeat-containing G protein-coupled receptor 7
UniProt Protein Name:	Relaxin receptor 1
UniProt Synonym Protein Names:	Leucine-rich repeat-containing G-protein coupled receptor 7; Relaxin family peptide receptor 1
Protein Family:	Relaxin receptor
UniProt Gene Name:	RXFP1
UniProt Entry Name:	RXFP1_HUMAN

Western blot analysis of extracts of various cell lines, using RXFP1 antibody (CAB7127) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

ELISA Kits

Human GPCR LGR7 / Relaxin R1 ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)