Antibody Name:	Anti-RUNX3 Antibody
Antibody SKU:	CAB7303
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 210-429 of human RUNX3 (NP_001026850.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	U-251MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 210-429 of human RUNX3 (NP_001026850.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DRFG DLER LRMR VTPS TPSP RGSL STTS HFSS QPQT PIQG TSEL NPFS DPRQ FDRS FPTL PTLT ESRF PDPR MHYP GAMS AAFP YSAT PSGT SISS LSVA GMPA TSRF HHTY LPPP YPGA PQNQ SGPF QANP SPYH LYYG TSSG SYQF SMVA GSSS GGDR SPTR MLAS CTSS AASV AAGN LMNP SLGG QSDG VEAD GSHS NSPT ALST PGRM DEAV WRPY
Gene ID:	864
Uniprot:	Q13761
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	44kDa/45kDa
Observed MW:	44kDa

Synonyms:

RUNX3, AML2, CBFA3, PEBP2aC

Background:

This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	AML2: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA- binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 1p36 Cellular Component: cytoplasm; nuclear chromatin; nucleus Molecular Function:protein binding; transcription factor activity Biological Process: chondrocyte differentiation; hemopoiesis; negative regulation of cell cycle; negative regulation of epithelial cell proliferation; negative regulation of transcription from RNA polymerase II promoter; ossification; peripheral nervous system neuron development; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; regulation of cell differentiation; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter
NCBI Summary:	This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
UniProt Code:	Q13761
NCBI GenInfo Identifier:	17368453
NCBI Gene ID:	864
NCBI Accession:	Q13761.2
UniProt Secondary Accession:	Q13761,Q12969, Q13760, B1AJV5,
UniProt Related Accession:	Q13761
Molecular Weight:	45,922 Da
NCBI Full Name:	Runt-related transcription factor 3
NCBI Synonym Full Names:	runt related transcription factor 3
NCBI Official Symbol:	RUNX3
NCBI Official Synonym Symbols:	AML2; CBFA3; PEBP2aC
NCBI Protein Information:	runt-related transcription factor 3
UniProt Protein Name:	Runt-related transcription factor 3
UniProt Synonym Protein Names:	Acute myeloid leukemia 2 protein; Core-binding factor subunit alpha-3; CBF-alpha-3; Oncogene AML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit; PEA2-alpha C; PEBP2-alpha C; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit
Protein Family:	Runt-related transcription factor
UniProt Gene Name:	RUNX3
UniProt Entry Name:	RUNX3_HUMAN

	Western blot analysis of extracts of U-251MG cells, using RUNX3 antibody (CAB7303) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunofluorescence analysis of MCF-7 cells using RUNX3 antibody (CAB7303). Blue: DAPI for nuclear staining.