Developmental Biology
Anti-RUNX2 Antibody (CAB2851)
- SKU:
- CAB2851
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-RUNX2 Antibody |
| Antibody SKU: | CAB2851 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 242-521 of human RUNX2 (NP_001019801.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:1000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | MCF7, HeLa, Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 242-521 of human RUNX2 (NP_001019801.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FSDR LSDL GRIP HPSM RVGV PPQN PRPS LNSA PSPF NPQG QSQI TDPR QAQS SPPW SYDQ SYPS YLSQ MTSP SIHS TTPL SSTR GTGL PAIT DVPR RISD DDTA TSDF CLWP STLS KKSQ AGAS ELGP FSDP RQFP SISS LTES RFSN PRMH YPAT FTYT PPVT SGMS LGMS ATTH YHTY LPPP YPGS SQSQ SGPF QTSS TPYL YYGT SSGS YQFP MVPG GDRS PSRM LPPC TTTS NGST LLNP NLPN QNDG VDAD GSHS SSPT VLNS SGRM DESV WRPY |
| Gene ID: | 860 |
| Uniprot: | Q13950 |
| Cellular Location: | Nucleus |
| Calculated MW: | 54kDa/55kDa/56kDa |
| Observed MW: | 60KDa |
| Synonyms: | RUNX2, AML3, CBF-alpha-1, CBFA1, CCD, CCD1, CLCD, OSF-2, OSF2, PEA2aA, PEBP2aA |
| Background: | This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Two regions of potential trinucleotide repeat expansions are present in the N-terminal region of the encoded protein, and these and other mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. |
| UniProt Protein Function: | AML3: Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'- PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Inhibits KAT6B-dependent transcriptional activation. Interaction with SATB2 results in enhanced DNA binding and transactivation by these transcription factors. Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3 and HIPK3. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80). Interacts with KAT6A and KAT6B. Binds to cyclin B1 CCNB1. Interacts with DDX5. Specifically expressed in osteoblasts. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 6p21 Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nuclear chromatin Molecular Function:protein domain specific binding; protein binding; bHLH transcription factor binding; chromatin binding; transcription factor activity; ATP binding Biological Process: embryonic forelimb morphogenesis; transcription initiation from RNA polymerase II promoter; ossification; positive regulation of transcription, DNA-dependent; cell maturation; regulation of fibroblast growth factor receptor signaling pathway; chondrocyte development; embryonic cranial skeleton morphogenesis; stem cell differentiation; osteoblast development; odontogenesis of dentine-containing teeth; BMP signaling pathway; osteoblast differentiation; positive regulation of osteoblast differentiation; positive regulation of chondrocyte differentiation; negative regulation of smoothened signaling pathway; positive regulation of cell proliferation; gene expression; negative regulation of transcription, DNA-dependent; regulation of odontogenesis of dentine-containing teeth; osteoblast fate commitment; T cell differentiation; endochondral ossification Disease: Metaphyseal Dysplasia With Maxillary Hypoplasia With Or Without Brachydactyly; Cleidocranial Dysplasia |
| NCBI Summary: | This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Two regions of potential trinucleotide repeat expansions are present in the N-terminal region of the encoded protein, and these and other mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2016] |
| UniProt Code: | Q13950 |
| NCBI GenInfo Identifier: | 17368460 |
| NCBI Gene ID: | 860 |
| NCBI Accession: | Q13950.2 |
| UniProt Related Accession: | Q13950 |
| Molecular Weight: | 56.6kDa |
| NCBI Full Name: | Runt-related transcription factor 2 |
| NCBI Synonym Full Names: | RUNX family transcription factor 2 |
| NCBI Official Symbol: | RUNX2 |
| NCBI Official Synonym Symbols: | CCD; AML3; CCD1; CLCD; OSF2; CBFA1; OSF-2; PEA2aA; PEBP2aA; CBF-alpha-1 |
| NCBI Protein Information: | runt-related transcription factor 2 |
| UniProt Protein Name: | Runt-related transcription factor 2 |
| UniProt Synonym Protein Names: | Acute myeloid leukemia 3 protein; Core-binding factor subunit alpha-1; CBF-alpha-1; Oncogene AML-3; Osteoblast-specific transcription factor 2; OSF-2; Polyomavirus enhancer-binding protein 2 alpha A subunit; PEA2-alpha A; PEBP2-alpha A; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit |
| Protein Family: | Runt-related transcription factor |
| UniProt Gene Name: | RUNX2 |
| UniProt Entry Name: | RUNX2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RUNX2 antibody (CAB2851) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Western blot analysis of extracts of Mouse testis, using RUNX2 antibody (CAB2851) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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