Epigenetics & Nuclear Signaling Antibodies 3
Anti-RUNX1T1 Antibody (CAB1737)
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- SKU:
- CAB1737
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-RUNX1T1 Antibody |
| Antibody SKU: | CAB1737 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-390 of human RUNX1T1 (NP_783554.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:1000 |
| Reactivity: | Human |
| Positive Samples: | HepG2, SH-SY5Y |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-390 of human RUNX1T1 (NP_783554.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GNDI SPEI GERV RTLV LGLV NSTL TIEE FHSK LQEA TNFP LRPF VIPF LKAN LPLL QREL LHCA RLAK QNPA QYLA QHEQ LLLD ASTT SPVD SSEL LLDV NENG KRRT PDRT KENG FDRE PLHS EHPS KRPC TISP GQRY SPNN GLSY QPNG LPHP TPPP PQHY RLDD MAIA HHYR DSYR HPSH RDLR DRNR PMGL HGTR QEEM IDHR LTDR EWAE EWKH LDHL LNCI MDMV EKTR RSLT VLRR CQEA DREE LNYW IRRY SDAE DLKK GGGS SSSH SRQQ SPVN PDPV ALD |
| Gene ID: | 862 |
| Uniprot: | Q06455 |
| Cellular Location: | Nucleus |
| Calculated MW: | 44kDa/48kDa/63kDa/64kDa/67kDa/68kDa |
| Observed MW: | 67kDa |
| Synonyms: | RUNX1T1, AML1-MTG8, AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2 |
| Background: | This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | RUNX1T1: Transcription regulator that excerts its function by binding to histone deacetylases and transcription factors. Can repress transactivation mediated by TCF12. Homotetramer. Heterotetramer with CBFA2T2 and CBFA2T3. Interacts with TCF12, SIN3A, HDAC1, HDAC2, HDAC3, NCOR1 and NCOR2. Interacts with ATN1 (via its N-terminus); the interaction enhances the transcriptional repression. Most abundantly expressed in brain. Lower levels in lung, heart, testis and ovary. Belongs to the CBFA2T family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription regulation; Oncoprotein Chromosomal Location of Human Ortholog: 8q22 Cellular Component: nucleoplasm; nuclear matrix; mitochondrion; cytoplasm Molecular Function:identical protein binding; protein binding; DNA binding; metal ion binding; transcription factor activity; transcription corepressor activity Biological Process: generation of precursor metabolites and energy; transcription, DNA-dependent; negative regulation of transcription, DNA-dependent |
| NCBI Summary: | This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010] |
| UniProt Code: | Q06455 |
| NCBI GenInfo Identifier: | 2498595 |
| NCBI Gene ID: | 862 |
| NCBI Accession: | Q06455.2 |
| UniProt Secondary Accession: | Q06455,Q61909, |
| UniProt Related Accession: | Q06455 |
| Molecular Weight: | 604 |
| NCBI Full Name: | Protein CBFA2T1 |
| NCBI Synonym Full Names: | runt-related transcription factor 1; translocated to, 1 (cyclin D-related) |
| NCBI Official Symbol: | RUNX1T1 |
| NCBI Official Synonym Symbols: | CDR; ETO; MTG8; AML1T1; ZMYND2; CBFA2T1 |
| NCBI Protein Information: | protein CBFA2T1; eight twenty one protein; CBFA2T1 isoform r1t1-7a47; CBFA2T1 isoform r1t1-7a48; CBFA2T1 isoform r1t1-7a49; CBFA2T1 isoform r1t1-7a50; CBFA2T1 isoform r1t1-7a52; CBFA2T1 isoform r1t1-7d53; CBFA2T1 isoform r1t1-7d54; CBFA2T1 isoform r1t1-7d |
| UniProt Protein Name: | Protein CBFA2T1 |
| UniProt Synonym Protein Names: | Cyclin-D-related protein; Eight twenty one protein; Protein ETO; Protein MTG8; Zinc finger MYND domain-containing protein 2 |
| Protein Family: | ETO1-like protein |
| UniProt Gene Name: | RUNX1T1 |
| UniProt Entry Name: | MTG8_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RUNX1T1 antibody (CAB1737) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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