Cell Biology Antibodies 11

Anti-RUFY1 Antibody (CAB7836)

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SKU:
CAB7836
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-RUFY1 Antibody
Antibody SKU:CAB7836
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 439-708 of human RUFY1 (NP_079434.3).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Mouse, Rat
Positive Samples:Mouse kidney, Mouse heart, Rat lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 439-708 of human RUFY1 (NP_079434.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EKDT HEKQ DTLV ALRQ QLEE VKAI NLQM FHKA QNAE SSLQ QKNE AITS FEGK TNQV MSSM KQME ERLQ HSER ARQG AEER SHKL QQEL GGRI GALQ LQLS QLHE QCSS LEKE LKSE KEQR QALQ RELQ HEKD TSSL LRME LQQV EGLK KELR ELQD EKAE LQKI CEEQ EQAL QEMG LHLS QSKL KMED IKEV NQAL KGHA WLKD DEAT HCRQ CEKE FSIS RRKH HCRN CGHI FCNT CSSN ELAL PSYP KPVR VCDS CHTL LLQR CSST AS
Gene ID:80230
Uniprot:Q96T51
Cellular Location:Cytoplasm, Early endosome membrane, Peripheral membrane protein
Calculated MW:44kDa/69kDa/79kDa
Observed MW:80kDa
Synonyms:RUFY1, RABIP4, ZFYVE12
Background:This gene encodes a protein that contains a RUN domain and a FYVE-type zinc finger domain. The encoded protein binds to phosphatidylinositol-3-phosphate (PI3P) and plays a role in early endosomal trafficking, tethering and fusion through interactions with small GTPases including Rab4, Rab5 and Rab14. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
UniProt Protein Function:RUFY1: Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: intracellular membrane-bound organelle; early endosome membrane; cytoplasm; nucleus

Molecular Function:protein binding; zinc ion binding; lipid binding; protein transporter activity

Biological Process: protein transport; regulation of endocytosis; endocytosis

NCBI Summary:This gene encodes a protein that contains a RUN domain and a FYVE-type zinc finger domain. The encoded protein binds to phosphatidylinositol-3-phosphate (PI3P) and plays a role in early endosomal trafficking, tethering and fusion through interactions with small GTPases including Rab4, Rab5 and Rab14. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
UniProt Code:Q96T51
NCBI GenInfo Identifier:94721332
NCBI Gene ID:80230
NCBI Accession:NP_001035541.1
UniProt Secondary Accession:Q96T51,Q59FF3, Q71S93, Q9H6I3,
UniProt Related Accession:Q96T51
Molecular Weight:79,818 Da
NCBI Full Name:RUN and FYVE domain-containing protein 1 isoform b
NCBI Synonym Full Names:RUN and FYVE domain containing 1
NCBI Official Symbol:RUFY1  
NCBI Official Synonym Symbols:RABIP4; ZFYVE12  
NCBI Protein Information:RUN and FYVE domain-containing protein 1; la-binding protein 1; FYVE-finger protein EIP1; rab4-interacting protein; zinc finger FYVE domain-containing protein 12
UniProt Protein Name:RUN and FYVE domain-containing protein 1
UniProt Synonym Protein Names:FYVE-finger protein EIP1; La-binding protein 1; Rab4-interacting protein; Zinc finger FYVE domain-containing protein 12
Protein Family:RUN and FYVE domain-containing protein
UniProt Gene Name:RUFY1  
UniProt Entry Name:RUFY1_HUMAN
Anti-RUFY1 Antibody (CAB7836)Western blot analysis of extracts of various cell lines, using RUFY1 antibody (CAB7836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-RUFY1 Antibody (CAB7836)Immunohistochemistry of paraffin-embedded rat brain using RUFY1 antibody (CAB7836) at dilution of 1:100 (40x lens).
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