Autophagy Antibodies

Anti-Rubicon Antibody (CAB13169)

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SKU:
CAB13169

Description

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Antibody Name:Anti-Rubicon Antibody
Antibody SKU:CAB13169
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Rubicon (NP_055502.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse
Positive Samples:Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Rubicon (NP_055502.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MRPE GAGM ELGG GEER LPEE SRRE HWQL LGNL KTTV EGLV STNS PNVW SKYG GLER LCRD MQSI LYHG LIRD QACR RQTD YWQF VKDI RWLS PHSA LHVE KFIS VHEN DQSS ADGA SERA VAEL WLQH SLQY HCLS AQLR PLLG DRQY IRKF YTDA AFLL SDAH VTAM LQCL EAVE QNNP RLLA QIDA SMFA RKHE SPLL
Gene ID:9711
Uniprot:Q92622
Cellular Location:Early endosome, Late endosome, Lysosome
Calculated MW:102kDa/103kDa/108kDa
Observed MW:108kDa
Synonyms:RUBCN, KIAA0226, RUBICON, SCAR15
Background:The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
UniProt Protein Function:rubicon: Negatively regulates endocytic trafficking. Impairs the autophagosome maturation process. May inhibit PIK3C3 activity. Forms a complex with BECN1, PIK3C3, PIK3R4 and UVRAG, but not with ATG14. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Unknown function

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: cytoplasm; early endosome; Golgi apparatus; intracellular membrane-bound organelle; late endosome; nucleoplasm

Molecular Function:protein binding

Biological Process: negative regulation of autophagy; negative regulation of endocytosis; negative regulation of phosphoinositide 3-kinase activity

Disease: Spinocerebellar Ataxia, Autosomal Recessive 15

NCBI Summary:The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014]
UniProt Code:Q92622
NCBI GenInfo Identifier:296439479
NCBI Gene ID:9711
NCBI Accession:Q92622.4
UniProt Secondary Accession:Q92622,Q96CK5,
UniProt Related Accession:Q92622
Molecular Weight:102,506 Da
NCBI Full Name:Run domain Beclin-1-interacting and cysteine-rich domain-containing protein
NCBI Synonym Full Names:RUN and cysteine rich domain containing beclin 1 interacting protein
NCBI Official Symbol:RUBCN  
NCBI Official Synonym Symbols:SCAR15; RUBICON; KIAA0226  
NCBI Protein Information:run domain Beclin-1-interacting and cysteine-rich domain-containing protein
UniProt Protein Name:Run domain Beclin-1-interacting and cysteine-rich domain-containing protein
UniProt Synonym Protein Names:Beclin-1 associated RUN domain containing protein; Baron
UniProt Gene Name:RUBCN  
UniProt Entry Name:RUBIC_HUMAN
Anti-Rubicon Antibody (CAB13169)Western blot analysis of extracts of mouse brain, using Rubicon antibody (CAB13169) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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Additional Information

Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Autophagy
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