Epigenetics & Nuclear Signaling Antibodies 1

Anti-RTCB Antibody (CAB10305)

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SKU:
CAB10305
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-RTCB Antibody
Antibody SKU:CAB10305
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 236-505 of human RTCB (NP_055121.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, Mouse liver, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 236-505 of human RTCB (NP_055121.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EIFN EYAA KKMG IDHK GQVC VMIH SGSR GLGH QVAT DALV AMEK AMKR DKII VNDR QLAC ARIA SPEG QDYL KGMA AAGN YAWV NRSS MTFL TRQA FAKV FNTT PDDL DLHV IYDV SHNI AKVE QHVV DGKE RTLL VHRK GSTR AFPP HHPL IAVD YQLT GQPV LIGG TMGT CSYV LTGT EQGM TETF GTTC HGAG RALS RAKS RRNL DFQD VLDK LADM GIAI RVAS PKLV MEEA PESY KNVT DVVN TCHD AGIS KKAI KLRP IAVI KG
Gene ID:51493
Uniprot:Q9Y3I0
Cellular Location:Cytoplasm, Nucleus
Calculated MW:55kDa
Observed MW:55kDa
Synonyms:RTCB, C22orf28, DJ149A16.6, FAAP, HSPC117
Background:
UniProt Protein Function:RTCB: Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'- phosphodiester. May act as a RNA ligase with broad substrate specificity, and may function toward other RNAs. Belongs to the RtcB family.
UniProt Protein Details:

Protein type:EC 6.5.1.3

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum membrane; intracellular membrane-bound organelle; nuclear envelope; nucleoplasm; nucleus

Molecular Function:protein binding; RNA binding; RNA ligase (ATP) activity; vinculin binding

Biological Process: tRNA splicing

UniProt Code:Q9Y3I0
NCBI GenInfo Identifier:74753486
NCBI Gene ID:51493
NCBI Accession:Q9Y3I0.1
UniProt Secondary Accession:Q9Y3I0,Q6IAI0, Q9BWL4, Q9NTH1, Q9P037, Q9P0J3, B2R6A8
UniProt Related Accession:Q9Y3I0
Molecular Weight:55kDa
NCBI Full Name:tRNA-splicing ligase RtcB homolog
NCBI Synonym Full Names:RNA 2',3'-cyclic phosphate and 5'-OH ligase
NCBI Official Symbol:RTCB  
NCBI Official Synonym Symbols:FAAP; HSPC117; C22orf28; DJ149A16.6  
NCBI Protein Information:tRNA-splicing ligase RtcB homolog
UniProt Protein Name:tRNA-splicing ligase RtcB homolog
Protein Family:RNA-splicing ligase
UniProt Gene Name:RTCB  
Anti-RTCB Antibody (CAB10305)Western blot analysis of extracts of various cell lines, using RTCB Antibody (CAB10305) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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