Immunology Antibodies 3

Anti-RSAD2 Antibody (CAB8271)

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SKU:
CAB8271
Product Type:
Antibody
Applications:
WB
Applications:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology

Description

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Antibody Name:RSAD2 Rabbit Polyclonal Antibody
Antibody SKU:CAB8271
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 42-361 of human RSAD2 (NP_542388.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:PC-3, Mouse spleen, Rat lung, Rat spleen, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 42-361 of human RSAD2 (NP_542388.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LATK RRKQ QLVL RGPD ETKE EEED PPLP TTPT SVNY HFTR QCNY KCGF CFHT AKTS FVLP LEEA KRGL LLLK EAGM EKIN FSGG EPFL QDRG EYLG KLVR FCKV ELRL PSVS IVSN GSLI RERW FQNY GEYL DILA ISCD SFDE EVNV LIGR GQGK KNHV ENLQ KLRR WCRD YRVA FKIN SVIN RFNV EEDM TEQI KALN PVRW KVFQ CLLI EGEN CGED ALRE AERF VIGD EEFE RFLE RHKE VSCL VPES NQKM KDSY LILD EYMR FLNC RKGR KDPS KSIL DVGV EEAI KFSG FDEK MFLK RGGK YIWS KADL KLDW
Gene ID:91543
Uniprot:Q8WXG1
Cellular Location:Cytoplasmic side, Endoplasmic reticulum, Endoplasmic reticulum membrane, Golgi apparatus, Lipid droplet, Mitochondrion, Mitochondrion inner membrane, Mitochondrion outer membrane, Peripheral membrane protein
Calculated MW:42kDa
Observed MW:46KDa
Synonyms:RSAD2, 2510004L01Rik, cig33, cig5, vig1
Background:
UniProt Protein Function:RSAD2: Interferon-inducible iron-sulfur (4FE-4S) cluster- binding antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. Can inhibit a wide range of DNA and RNA viruses, including human cytomegalovirus (HCMV), hepatitis C virus (HCV), west Nile virus (WNV), dengue virus, sindbis virus, influenza A virus, sendai virus, vesicular stomatitis virus (VSV), and human immunodeficiency virus (HIV-1). Displays antiviral activity against influenza A virus by inhibiting the budding of the virus from the plasma membrane by disturbing the lipid rafts. This is accomplished, at least in part, through binding and inhibition of the enzyme farnesyl diphospate synthase (FPPS), which is essential for the biosynthesis of isoprenoid-derived lipids. Promotes TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating Lys-63'-linked ubiquitination of IRAK1. Plays a role in CD4+ T-cells activation and differentiation. Facilitates T-cell receptor (TCR)-mediated GATA3 activation and optimal T helper 2 (Th2) cytokine production by modulating NFKB1 and JUNB activities. Can inhibit secretion of soluble proteins. Belongs to the radical SAM superfamily. RSAD2 family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 2p25.2

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; Golgi apparatus; lipid particle; mitochondrial inner membrane; mitochondrial outer membrane; mitochondrion

Molecular Function:4 iron, 4 sulfur cluster binding; catalytic activity; metal ion binding; protein binding; protein self-association

Biological Process: CD4-positive, alpha beta T cell differentiation; cytokine and chemokine mediated signaling pathway; defense response to virus; metabolic process; negative regulation of protein secretion; negative regulation of viral genome replication; positive regulation of toll-like receptor 7 signaling pathway; positive regulation of toll-like receptor 9 signaling pathway; response to virus; viral reproduction

UniProt Code:Q8WXG1
NCBI GenInfo Identifier:74724033
NCBI Gene ID:91543
NCBI Accession:Q8WXG1.1
UniProt Secondary Accession:Q8WXG1,Q8WVI4,
UniProt Related Accession:Q8WXG1
Molecular Weight:42,170 Da
NCBI Full Name:Radical S-adenosyl methionine domain-containing protein 2
NCBI Synonym Full Names:radical S-adenosyl methionine domain containing 2
NCBI Official Symbol:RSAD2  
NCBI Official Synonym Symbols:cig5; vig1; cig33; 2510004L01Rik  
NCBI Protein Information:radical S-adenosyl methionine domain-containing protein 2
UniProt Protein Name:Radical S-adenosyl methionine domain-containing protein 2
UniProt Synonym Protein Names:Cytomegalovirus-induced gene 5 protein; Viperin; Virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible
Protein Family:Radical S-adenosyl methionine domain-containing protein
UniProt Gene Name:RSAD2  
UniProt Entry Name:RSAD2_HUMAN
Anti-RSAD2 Polyclonal Antibody (CAB8271)Western blot analysis of extracts of various cell lines, using RSAD2 antibody (CAB8271) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
Anti-RSAD2 Polyclonal Antibody (CAB8271)Immunofluorescence analysis of U2OS cells using RSAD2 antibody (CAB8271) at dilution of 1:100. Blue: DAPI for nuclear staining.
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