Cell Biology Antibodies 4

Anti-RRP36 Antibody (CAB14328)

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SKU:
CAB14328
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-RRP36 Antibody
Antibody SKU:CAB14328
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-259 of human RRP36 (NP_149103.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:MCF7, 293T, BxPC-3, HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-259 of human RRP36 (NP_149103.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPGA NYRA GAGA GAGA RRPR GARD REED GGGL EPAA VARD LLRG TSNM SFEE LLEL QSQV GTKT YKQL VAGN SPKK QASR PPIQ NACV ADKH RPLE MSAK IRVP FLRQ VVPI SKKV ARDP RFDD LSGE YNPE VFDK TYQF LNDI RAKE KELV KKQL KKHL SGEE HEKL QQLL QRME QQEM AQQE RKQQ QELH LALK QERR AQAQ QGHR PYFL KKSE QRQL ALAE KFKE LKRS KKLE NFLS RKRR RNAG KDRR HLPL SKE
Gene ID:88745
Uniprot:Q96EU6
Cellular Location:Nucleus, nucleolus
Calculated MW:29kDa
Observed MW:36kDa
Synonyms:RRP36, C6orf153, dJ20C7.4
Background:
UniProt Protein Function:RRP36: Involved in the early processing steps of the pre-rRNA in the maturation pathway leading to the 18S rRNA. Belongs to the RRP36 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Nucleolus; RNA-binding

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: nucleolus; nucleoplasm; nucleus

Biological Process: maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); ribosomal small subunit biogenesis and assembly; rRNA processing

NCBI Summary:RRP36 functions at an early stage in the processing of 35S preribosomal RNA into the mature 18S species (Gerus et al., 2010 [PubMed 20038530]).[supplied by OMIM, Jul 2010]
UniProt Code:Q96EU6
NCBI GenInfo Identifier:74751848
NCBI Gene ID:88745
NCBI Accession:Q96EU6.1
UniProt Secondary Accession:Q96EU6,Q9BRF6, Q9P0C8,
UniProt Related Accession:Q96EU6
Molecular Weight:29,129 Da
NCBI Full Name:Ribosomal RNA processing protein 36 homolog
NCBI Synonym Full Names:ribosomal RNA processing 36
NCBI Official Symbol:RRP36  
NCBI Official Synonym Symbols:C6orf153; dJ20C7.4  
NCBI Protein Information:ribosomal RNA processing protein 36 homolog
UniProt Protein Name:Ribosomal RNA processing protein 36 homolog
Protein Family:rRNA biogenesis protein
UniProt Gene Name:RRP36  
UniProt Entry Name:RRP36_HUMAN
Anti-RRP36 Antibody (CAB14328)Western blot analysis of extracts of various cell lines, using RRP36 antibody (CAB14328) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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