Epigenetics & Nuclear Signaling Antibodies 3

Anti-RRM2 Antibody (CAB5255)

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SKU:
CAB5255
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-RRM2 Antibody
Antibody SKU:CAB5255
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-389 of human RRM2 (NP_001025.1).
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:10 - 1:100 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:MCF-7, SW480, HeLa, Jurkat, Mouse thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-389 of human RRM2 (NP_001025.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MLSL RVPL APIT DPQQ LQLS PLKG LSLV DKEN TPPA LSGT RVLA SKTA RRIF QEPT EPKT KAAA PGVE DEPL LREN PRRF VIFP IEYH DIWQ MYKK AEAS FWTA EEVD LSKD IQHW ESLK PEER YFIS HVLA FFAA SDGI VNEN LVER FSQE VQIT EARC FYGF QIAM ENIH SEMY SLLI DTYI KDPK EREF LFNA IETM PCVK KKAD WALR WIGD KEAT YGER VVAF AAVE GIFF SGSF ASIF WLKK RGLM PGLT FSNE LISR DEGL HCDF ACLM FKHL VHKP SEER VREI IINA VRIE QEFL TEAL PVKL IGMN CTLM KQYI EFVA DRLM LELG FSKV FRVE NPFD FMEN ISLE GKTN FFEK RVGE YQRM GVMS SPTE NSFT LDAD F
Gene ID:6241
Uniprot:P31350
Cellular Location:Cytoplasm
Calculated MW:44kDa/51kDa
Observed MW:45kDa
Synonyms:RRM2, R2, RR2, RR2M
Background:This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. Transcription from this gene can initiate from alternative promoters, which results in two isoforms that differ in the lengths of their N-termini. Related pseudogenes have been identified on chromosomes 1 and X.
UniProt Protein Function:RRM2: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling. Belongs to the ribonucleoside diphosphate reductase small chain family.
UniProt Protein Details:

Protein type:Other Amino Acids Metabolism - glutathione; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; EC 1.17.4.1; Oxidoreductase

Chromosomal Location of Human Ortholog: 2p25-p24

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function:protein binding; metal ion binding; ribonucleoside-diphosphate reductase activity

Biological Process: G1/S-specific transcription in mitotic cell cycle; deoxyribonucleoside diphosphate metabolic process; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; deoxyribonucleotide biosynthetic process; protein heterotetramerization; mitotic cell cycle; DNA replication; G1/S transition of mitotic cell cycle

NCBI Summary:This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. Transcription from this gene can initiate from alternative promoters, which results in two isoforms that differ in the lengths of their N-termini. Related pseudogenes have been identified on chromosomes 1 and X. [provided by RefSeq, Sep 2009]
UniProt Code:P31350
NCBI GenInfo Identifier:400979
NCBI Gene ID:6241
NCBI Accession:P31350.1
UniProt Secondary Accession:P31350,Q5WRU7, B2R9B5, J3KP43,
UniProt Related Accession:P31350
Molecular Weight:389
NCBI Full Name:Ribonucleoside-diphosphate reductase subunit M2
NCBI Synonym Full Names:ribonucleotide reductase M2
NCBI Official Symbol:RRM2  
NCBI Official Synonym Symbols:R2; RR2; RR2M  
NCBI Protein Information:ribonucleoside-diphosphate reductase subunit M2; ribonucleotide reductase small chain; ribonucleotide reductase small subunit; ribonucleotide reductase M2 polypeptide
UniProt Protein Name:Ribonucleoside-diphosphate reductase subunit M2
UniProt Synonym Protein Names:Ribonucleotide reductase small chain; Ribonucleotide reductase small subunit
Protein Family:Ribonucleoside-diphosphate reductase
UniProt Gene Name:RRM2  
UniProt Entry Name:RIR2_HUMAN
Anti-RRM2 Antibody (CAB5255)Western blot analysis of extracts of various cell lines, using RRM2 antibody (CAB5255) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-RRM2 Antibody (CAB5255)Immunofluorescence analysis of A549 cells using RRM2 antibody (CAB5255). Blue: DAPI for nuclear staining.
Anti-RRM2 Antibody (CAB5255)Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug RRM2 antibody (CAB5255). Western blot was performed from the immunoprecipitate using RRM2 antibody (CAB5255) at a dilition of 1:1000.
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