Name: Anti-RRAS2 Antibody (CAB7076)
Brand: Cell Biology Antibodies 10
SKU: CAB7076
Availability: OutOfStock

Antibody Name:	Anti-RRAS2 Antibody
Antibody SKU:	CAB7076
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-204A of human RRAS2 (NP_036382.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, SKOV3, NIH/3T3, Mouse kidney, Mouse heart, Mouse lung, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-204A of human RRAS2 (NP_036382.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAA GWRD GSGQ EKYR LVVV GGGG VGKS ALTI QFIQ SYFV TDYD PTIE DSYT KQCV IDDR AARL DILD TAGQ EEFG AMRE QYMR TGEG FLLV FSVT DRGS FEEI YKFQ RQIL RVKD RDEF PMIL IGNK ADLD HQRQ VTQE EGQQ LARQ LKVT YMEA SAKI RMNV DQAF HELV RVIR KFQE QECP PSPE PTRK EKDK KGCH CVIF
Gene ID:	22800
Uniprot:	P62070
Cellular Location:	Cell membrane, Cytoplasmic side, Lipid-anchor
Calculated MW:	15kDa/19kDa/23kDa/24kDa
Observed MW:	23kDa

Synonyms:

RRAS2, TC21

Background:

This gene encodes a member of the R-Ras subfamily of Ras-like small GTPases. The encoded protein associates with the plasma membrane and may function as a signal transducer. This protein may play an important role in activating signal transduction pathways that control cell proliferation. Mutations in this gene are associated with the growth of certain tumors. Pseudogenes of this gene are found on chromosomes 1 and 2. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	RRAS2: It is a plasma membrane-associated GTP-binding protein with GTPase activity. Might transduce growth inhibitory signals across the cell membrane, exerting its effect through an effector shared with the Ras proteins but in an antagonistic fashion. Defects in RRAS2 are a cause of susceptibility to ovarian cancer (OC). The term ovarian cancer defines common malignancies originating from ovarian tissue. Although many histologic types of ovarian tumors have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease. Belongs to the small GTPase superfamily. Ras family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:G protein; Oncoprotein; G protein, monomeric, Ras; G protein, monomeric; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 11p15.2 Cellular Component: focal adhesion; membrane; endoplasmic reticulum; plasma membrane Molecular Function:GTPase activity; protein binding; GTP binding Biological Process: osteoblast differentiation; metabolic process; Ras protein signal transduction; positive regulation of cell migration
NCBI Summary:	This gene encodes a member of the R-Ras subfamily of Ras-like small GTPases. The encoded protein associates with the plasma membrane and may function as a signal transducer. This protein may play an important role in activating signal transduction pathways that control cell proliferation. Mutations in this gene are associated with the growth of certain tumors. Pseudogenes of this gene are found on chromosomes 1 and 2. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]
UniProt Code:	P62070
NCBI GenInfo Identifier:	49065833
NCBI Gene ID:	22800
NCBI Accession:	P62070.1
UniProt Secondary Accession:	P62070,P17082, B2R9Z3, B7Z5Z2, B7Z6C4, B7Z7H6,
UniProt Related Accession:	P62070
Molecular Weight:	204
NCBI Full Name:	Ras-related protein R-Ras2
NCBI Synonym Full Names:	related RAS viral (r-ras) oncogene homolog 2
NCBI Official Symbol:	RRAS2
NCBI Official Synonym Symbols:	TC21
NCBI Protein Information:	ras-related protein R-Ras2; ras-like protein TC21; teratocarcinoma oncogene
UniProt Protein Name:	Ras-related protein R-Ras2
UniProt Synonym Protein Names:	Ras-like protein TC21; Teratocarcinoma oncogene
Protein Family:	Ras-related protein
UniProt Gene Name:	RRAS2
UniProt Entry Name:	RRAS2_HUMAN

Western blot analysis of extracts of various cell lines, using RRAS2 antibody (CAB7076) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

RRAS2 Antibody (PACO11967)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)