Cell Biology Antibodies 12

Anti-RRAGD Antibody (CAB9979)

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SKU:
CAB9979
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-RRAGD Antibody
Antibody SKU:CAB9979
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 181-400 of human RRAGD (NP_067067.1).
Application:WB IHC
Recommended Dilution:WB 1:1000 - 1:4000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, NCI-H460, SW480, HL-60, 22Rv1, Mouse heart, Mouse skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 181-400 of human RRAGD (NP_067067.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VDGL SDDH KIET QRDI HQRA NDDL ADAG LEKI HLSF YLTS IYDH SIFE AFSK VVQK LIPQ LPTL ENLL NIFI SNSG IEKA FLFD VVSK IYIA TDST PVDM QTYE LCCD MIDV VIDI SCIY GLKE DGAG TPYD KEST AIIK LNNT TVLY LKEV TKFL ALVC FVRE ESFE RKGL IDYN FHCF RKAI HEVF EVRM KVVK SRKV QNRL QKKK RATP NGTP RVLL
Gene ID:58528
Uniprot:Q9NQL2
Cellular Location:Cytoplasm, Lysosome, Nucleus
Calculated MW:28kDa/45kDa
Observed MW:46kDa
Synonyms:RRAGD, RAGD, bA11D8.2.1
Background:
UniProt Protein Function:RRAGD: Has guanine nucleotide-binding activity but lacks intrinsic GTPase activity. Probably required for the amino acid- induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Forms a heterodimer with RRAGA in a sequence-independent manner. Binds GTP. Interacts with NOL8 and RRAGB. Belongs to the GTR/RAG GTP-binding protein family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:G protein regulator, misc.

Chromosomal Location of Human Ortholog: 6q15

Cellular Component: cytoplasm; cytosol; lysosome; nucleus

Molecular Function:GTPase activity; protein binding; protein heterodimerization activity

Biological Process: cell cycle arrest; cellular response to starvation; macroautophagy; positive regulation of TOR signaling pathway; regulation of autophagy

NCBI Summary:RRAGD is a monomeric guanine nucleotide-binding protein, or G protein. By binding GTP or GDP, small G proteins act as molecular switches in numerous cell processes and signaling pathways.[supplied by OMIM, Apr 2004]
UniProt Code:Q9NQL2
NCBI GenInfo Identifier:74752904
NCBI Gene ID:58528
NCBI Accession:Q9NQL2.1
UniProt Secondary Accession:Q9NQL2,Q7L8F9, Q9NPG0, A8K184,
UniProt Related Accession:Q9NQL2
Molecular Weight:28,611 Da
NCBI Full Name:Ras-related GTP-binding protein D
NCBI Synonym Full Names:Ras related GTP binding D
NCBI Official Symbol:RRAGD  
NCBI Official Synonym Symbols:RAGD; bA11D8.2.1  
NCBI Protein Information:ras-related GTP-binding protein D
UniProt Protein Name:Ras-related GTP-binding protein D
UniProt Gene Name:RRAGD  
UniProt Entry Name:RRAGD_HUMAN
Anti-RRAGD Polyclonal Antibody (CAB9979)Western blot analysis of extracts of various cell lines, using RRAGD antibody (CAB9979) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-RRAGD Polyclonal Antibody (CAB9979)Immunohistochemistry of paraffin-embedded human liver cancer using RRAGD antibody (CAB9979) at dilution of 1:100 (40x lens).
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