Cell Biology Antibodies 6
Anti-RPS6KB2 Antibody (CAB1541)
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- SKU:
- CAB1541
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-RPS6KB2 Antibody |
| Antibody SKU: | CAB1541 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human RPS6KB2 (NP_003943.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:1000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, SH-SY5Y, HL-60, SW620, MCF7, Mouse liver, Mouse kidney, Mouse spleen, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human RPS6KB2 (NP_003943.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAV FDLD LETE EGSE GEGE PELS PADA CPLA ELRA AGLE PVGH YEEV ELTE TSVN VGPE RIGP HCFE LLRV LGKG GYGK VFQV RKVQ GTNL GKIY AMKV LRKA KIVR NAKD TAHT RAER NILE SVKH PFIV ELAY AFQT GGKL YLIL ECLS GGEL FTHL EREG IFLE DT |
| Gene ID: | 6199 |
| Uniprot: | Q9UBS0 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 16kDa/53kDa |
| Observed MW: | 63kDa |
| Synonyms: | RPS6KB2, KLS, P70-beta, P70-beta-1, P70-beta-2, S6K-beta2, S6K2, SRK, STK14B, p70(S6K)-beta |
| Background: | This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains a kinase catalytic domain and phosphorylates the S6 ribosomal protein and eukaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation. |
| UniProt Protein Function: | P70S6KB: an AGC kinase of the RSK family that is required for cell growth and G1 cell cycle progression. Is phosphorylated and activated by mTOR in mitogenic pathways downstream of phosphoinositide 3 kinase (PI3K). Phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. Activity is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains. Mouse knockout shows symptoms of insulin resistance, and increased insulin senstivity, resulting in protection against diet-induced obesity. Protein expression and activation upregulated in colon adenocarcinoma cell lines. Increased expression in breast cancer correlated with poor survival. Selectively amplified and overexpressed within the 17q23 breast cancer amplicon. Two isoforms produced by alternative initiation have been reported. |
| UniProt Protein Details: | Protein type:EC 2.7.11.1; Protein kinase, AGC; Translation; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; AGC group; RSK family; p70 subfamily Chromosomal Location of Human Ortholog: 11q13.2 Cellular Component: cytoplasm; nucleoplasm Molecular Function:ATP binding; peptide binding; protein kinase activity; protein serine/threonine kinase activity; ribosomal protein S6 kinase activity Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of translational initiation; protein amino acid phosphorylation; protein kinase B signaling cascade; signal transduction; translation |
| NCBI Summary: | This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains a kinase catalytic domain and phosphorylates the S6 ribosomal protein and eukaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation. [provided by RefSeq, Jan 2015] |
| UniProt Code: | Q9UBS0 |
| NCBI GenInfo Identifier: | 296434560 |
| NCBI Gene ID: | 6199 |
| NCBI Accession: | Q9UBS0.2 |
| UniProt Secondary Accession: | Q9UBS0,O94809, Q9UEC1, B2RMZ9, B4DML8, |
| UniProt Related Accession: | Q9UBS0 |
| Molecular Weight: | 16,868 Da |
| NCBI Full Name: | Ribosomal protein S6 kinase beta-2 |
| NCBI Synonym Full Names: | ribosomal protein S6 kinase B2 |
| NCBI Official Symbol: | RPS6KB2 |
| NCBI Official Synonym Symbols: | KLS; SRK; S6K2; STK14B; p70S6Kb; P70-beta; S6K-beta2; P70-beta-1; P70-beta-2; p70(S6K)-beta |
| NCBI Protein Information: | ribosomal protein S6 kinase beta-2 |
| UniProt Protein Name: | Ribosomal protein S6 kinase beta-2 |
| UniProt Synonym Protein Names: | 70 kDa ribosomal protein S6 kinase 2; P70S6K2; p70-S6K 2; S6 kinase-related kinase; SRK; Serine/threonine-protein kinase 14B; p70 ribosomal S6 kinase beta; S6K-beta; p70 S6 kinase beta; p70 S6K-beta; p70 S6KB; p70-beta |
| Protein Family: | Ribosomal protein S6 kinase |
| UniProt Gene Name: | RPS6KB2 |
| UniProt Entry Name: | KS6B2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RPS6KB2 antibody (CAB1541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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