Antibody Name:	Anti-RPS6KA4 Antibody
Antibody SKU:	CAB10130
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human RPS6KA4 (NP_003933.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:1000 IHC 1:20 - 1:100 IF 1:20 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, SH-SY5Y, HL-60, BT-474, Mouse heart, Mouse kidney, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human RPS6KA4 (NP_003933.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGDE DDDE SCAV ELRI TEAN LTGH EEKV SVEN FELL KVLG TGAY GKVF LVRK AGGH DAGK LYAM KVLR KAAL VQRA KTQE HTRT ERSV LELV RQAP FLVT LHYA FQTD AKLH LILD YVSG GEMF THLY QRQY FKEA EVRV YGGE IVLA LEHL HKLG IIYR DLKL ENVL LDSE GHIV LTDF GLSK EFLT EEKE RTFS FCGT IEYM APEI IRSK TGHG KAVD
Gene ID:	8986
Uniprot:	O75676
Cellular Location:	Nucleus
Calculated MW:	84kDa/85kDa
Observed MW:	115kDa

Synonyms:

RPS6KA4, MSK2, RSK-B, S6K-alpha-4

Background:

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and ATF1. The encoded protein can also phosphorylate histone H3 to regulate certain inflammatory genes. Several transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	MSK2: an AGC kinase of the RSK family. Has two kinase domains connected by a regulatory linker region. Phosphorylates H3 and HMG-14 in response to growth factors and cellular stress.
UniProt Protein Details:	Protein type:Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; EC 2.7.11.1; Kinase, protein; AGC group; RSK family; MSK subfamily Chromosomal Location of Human Ortholog: 11q11-q13 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:ATP binding; histone serine kinase activity (H3-S10 specific); mitogen-activated protein kinase p38 binding; protein binding; protein serine/threonine kinase activity Biological Process: activation of CREB transcription factor; activation of NF-kappaB transcription factor; histone phosphorylation; negative regulation of cytokine production; positive regulation of histone acetylation; positive regulation of histone phosphorylation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of transcription, DNA-dependent
NCBI Summary:	This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and ATF1. The encoded protein can also phosphorylate histone H3 to regulate certain inflammatory genes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]
UniProt Code:	O75676
NCBI GenInfo Identifier:	37999483
NCBI Gene ID:	8986
NCBI Accession:	O75676.1
UniProt Secondary Accession:	O75676,O75585, Q53ES8, A8K7Z8,
UniProt Related Accession:	O75676
Molecular Weight:	84,884 Da
NCBI Full Name:	Ribosomal protein S6 kinase alpha-4
NCBI Synonym Full Names:	ribosomal protein S6 kinase A4
NCBI Official Symbol:	RPS6KA4
NCBI Official Synonym Symbols:	MSK2; RSK-B; S6K-alpha-4
NCBI Protein Information:	ribosomal protein S6 kinase alpha-4
UniProt Protein Name:	Ribosomal protein S6 kinase alpha-4
UniProt Synonym Protein Names:	90 kDa ribosomal protein S6 kinase 4; Nuclear mitogen- and stress-activated protein kinase 2; Ribosomal protein kinase B; RSKB
Protein Family:	Ribosomal protein S6 kinase
UniProt Gene Name:	RPS6KA4
UniProt Entry Name:	KS6A4_HUMAN

	Western blot analysis of extracts of various cell lines, using RPS6KA4 antibody (CAB10130) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded mouse lung using RPS6KA4 antibody (CAB10130) at dilution of 1:200 (40x lens).