Antibody Name:	Anti-RPS6KA3 Antibody
Antibody SKU:	CAB5436
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 68-327 of human RPS6KA3 (NP_004577.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:50
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 68-327 of human RPS6KA3 (NP_004577.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FELL KVLG QGSF GKVF LVKK ISGS DARQ LYAM KVLK KATL KVRD RVRT KMER DILV EVNH PFIV KLHY AFQT EGKL YLIL DFLR GGDL FTRL SKEV MFTE EDVK FYLA ELAL ALDH LHSL GIIY RDLK PENI LLDE EGHI KLTD FGLS KESI DHEK KAYS FCGT VEYM APEV VNRR GHTQ SADW WSFG VLMF EMLT GTLP FQGK DRKE TMTM ILKA KLGM PQFL SPEA QSLL RMLF KRNP ANRL GAGP DGVE EIKR HSFF
Gene ID:	6197
Uniprot:	P51812
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	83kDa
Observed MW:	84kDa

Synonyms:

RPS6KA3, CLS, HU-3, ISPK-1, MAPKAPK1B, MRX19, RSK, RSK2, S6K-alpha3, p90-RSK2, pp90RSK2

Background:

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS).

UniProt Protein Function:	RSK2: an AGC kinase of the RSK family. Phosphorylated and activated by Erk1 and -2 in response to many growth factors, polypeptide hormones and neurotransmitters. Several phosphorylation sites are important for its activation. Possesses two kinase domains connected by a regulator linker region. Phosphorylates a wide range of substrates including ribosomal protein S6. Prominently expressed in brain structures essential for cognitive function and learning.
UniProt Protein Details:	Protein type:Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, AGC; AGC group; RSK family; RSK subfamily Chromosomal Location of Human Ortholog: Xp22.2-p22.1 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus Molecular Function:caspase inhibitor activity; kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity Biological Process: central nervous system development; negative regulation of apoptosis; negative regulation of caspase activity; positive regulation of cell differentiation; positive regulation of cell growth; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription in response to stress; regulation of translation in response to stress; response to lipopolysaccharide; signal transduction; skeletal development; toll-like receptor signaling pathway Disease: Coffin-lowry Syndrome; Mental Retardation, X-linked 19
NCBI Summary:	This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq, Jul 2008]
UniProt Code:	P51812
NCBI GenInfo Identifier:	1730070
NCBI Gene ID:	6197
NCBI Accession:	P51812.1
UniProt Secondary Accession:	P51812,Q4VAP3, Q59H26, Q5JPK8, Q7Z3Z7, B2R9V4,
UniProt Related Accession:	P51812
Molecular Weight:	83,736 Da
NCBI Full Name:	Ribosomal protein S6 kinase alpha-3
NCBI Synonym Full Names:	ribosomal protein S6 kinase A3
NCBI Official Symbol:	RPS6KA3
NCBI Official Synonym Symbols:	CLS; RSK; HU-3; RSK2; MRX19; ISPK-1; p90-RSK2; pp90RSK2; MAPKAPK1B; S6K-alpha3
NCBI Protein Information:	ribosomal protein S6 kinase alpha-3
UniProt Protein Name:	Ribosomal protein S6 kinase alpha-3
UniProt Synonym Protein Names:	90 kDa ribosomal protein S6 kinase 3; p90-RSK 3; p90RSK3; Insulin-stimulated protein kinase 1; ISPK-1; MAP kinase-activated protein kinase 1b; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK-1b; Ribosomal S6 kinase 2; RSK-2; pp90RSK2
Protein Family:	Ribosomal protein S6 kinase
UniProt Gene Name:	RPS6KA3
UniProt Entry Name:	KS6A3_HUMAN

	Western blot analysis of extracts of mouse lung, using RPS6KA3 antibody (CAB5436) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using RPS6KA3 antibody (CAB5436) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using RPS6KA3 antibody (CAB5436) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse stomach using RPS6KA3 antibody (CAB5436) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using RPS6KA3 antibody (CAB5436). Blue: DAPI for nuclear staining.