Antibody Name:	Anti-RPS4X Antibody
Antibody SKU:	CAB6730
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 66-263 of human RPS4X (NP_000998.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, Mouse spleen, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 66-263 of human RPS4X (NP_000998.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MQRF IKID GKVR TDIT YPAG FMDV ISID KTGE NFRL IYDT KGRF AVHR ITPE EAKY KLCK VRKI FVGT KGIP HLVT HDAR TIRY PDPL IKVN DTIQ IDLE TGKI TDFI KFDT GNLC MVTG GANL GRIG VITN RERH PGSF DVVH VKDA NGNS FATR LSNI FVIG KGNK PWIS LPRG KGIR LTIA EERD KRLA AKQS SG
Gene ID:	6191
Uniprot:	P62701
Cellular Location:	Cytoplasm
Calculated MW:	29kDa
Observed MW:	30KDa

Synonyms:

RPS4X, CCG2, DXS306, RPS4, S4, SCAR, SCR10, X-linked

Background:

Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes ribosomal protein S4, a component of the 40S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene and ribosomal protein S4, Y-linked (RPS4Y). The 2 isoforms encoded by these genes are not identical, but are functionally equivalent. Ribosomal protein S4 belongs to the S4E family of ribosomal proteins. This gene is not subject to X-inactivation. It has been suggested that haploinsufficiency of the ribosomal protein S4 genes plays a role in Turner syndrome; however, this hypothesis is controversial. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	RPS4X: Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes ribosomal protein S4, a component of the 40S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene and ribosomal protein S4, Y-linked (RPS4Y). The 2 isoforms encoded by these genes are not identical, but are functionally equivalent. Ribosomal protein S4 belongs to the S4E family of ribosomal proteins. This gene is not subject to X-inactivation. It has been suggested that haploinsufficiency of the ribosomal protein S4 genes plays a role in Turner syndrome; however, this hypothesis is controversial. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Ribosomal; Translation; RNA-binding Chromosomal Location of Human Ortholog: Xq13.1 Cellular Component: cytosol; focal adhesion; membrane; nucleoplasm; polysome; ribonucleoprotein complex; ribosome; small ribosomal subunit Molecular Function:protein binding; structural constituent of ribosome Biological Process: mRNA catabolic process, nonsense-mediated decay; multicellular organismal development; positive regulation of cell proliferation; positive regulation of translation; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translation; translational initiation; viral transcription
NCBI Summary:	Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes ribosomal protein S4, a component of the 40S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene and ribosomal protein S4, Y-linked (RPS4Y). The 2 isoforms encoded by these genes are not identical, but are functionally equivalent. Ribosomal protein S4 belongs to the S4E family of ribosomal proteins. This gene is not subject to X-inactivation. It has been suggested that haploinsufficiency of the ribosomal protein S4 genes plays a role in Turner syndrome; however, this hypothesis is controversial. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	P62701
NCBI GenInfo Identifier:	50403628
NCBI Gene ID:	6191
NCBI Accession:	P62701.2
UniProt Secondary Accession:	P62701,P12631, P12750, P27576, P55831, Q14727, Q6IPY4
UniProt Related Accession:	P62701
Molecular Weight:	29,598 Da
NCBI Full Name:	40S ribosomal protein S4, X isoform
NCBI Synonym Full Names:	ribosomal protein S4, X-linked
NCBI Official Symbol:	RPS4X
NCBI Official Synonym Symbols:	S4; CCG2; RPS4; SCAR; SCR10; DXS306
NCBI Protein Information:	40S ribosomal protein S4, X isoform
UniProt Protein Name:	40S ribosomal protein S4, X isoform
UniProt Synonym Protein Names:	SCR10; Single copy abundant mRNA protein
Protein Family:	40S ribosomal protein
UniProt Gene Name:	RPS4X
UniProt Entry Name:	RS4X_HUMAN

	Western blot analysis of extracts of various cell lines, using RPS4X antibody (CAB6730) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human kidney using RPS4X antibody (CAB6730) at dilution of 1:100 (40x lens).