Antibody Name:	Anti-RPS25 Antibody
Antibody SKU:	CAB15314
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human RPS25 (NP_001019.1).

Application:	WB IF
Recommended Dilution:	WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A431, BxPC-3, A549, HeLa, K-562, Mouse liver, Mouse pancreas, Rat liver

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human RPS25 (NP_001019.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FDKA TYDK LCKE VPNY KLIT PAVV SERL KIRG SLAR AALQ ELLS KGLI KLVS KHRA QVIY TRNT KGGD APAA GEDA
Gene ID:	6230
Uniprot:	P62851
Cellular Location:
Calculated MW:	13kDa
Observed MW:	14kDa

Synonyms:

RPS25, S25

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S25E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	RPS25: a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S25E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Ribosomal; Translation Chromosomal Location of Human Ortholog: 11q23.3 Cellular Component: cytosol; extracellular matrix; nucleolus; nucleoplasm; nucleus; ribosome; small ribosomal subunit Molecular Function:protein binding; RNA binding Biological Process: mRNA catabolic process, nonsense-mediated decay; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translation; translational initiation; viral transcription
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S25E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	P62851
NCBI GenInfo Identifier:	4506707
NCBI Gene ID:	6230
NCBI Accession:	NP_001019.1
UniProt Secondary Accession:	P62851,P25111, B2R4M7,
UniProt Related Accession:	P62851
Molecular Weight:	41.1kD
NCBI Full Name:	40S ribosomal protein S25
NCBI Synonym Full Names:	ribosomal protein S25
NCBI Official Symbol:	RPS25
NCBI Official Synonym Symbols:	S25
NCBI Protein Information:	40S ribosomal protein S25
UniProt Protein Name:	40S ribosomal protein S25
UniProt Synonym Protein Names:	Small ribosomal subunit protein eS25
Protein Family:	40S ribosomal protein
UniProt Gene Name:	RPS25

	Western blot analysis of extracts of various cell lines, using RPS25 antibody (CAB15314) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunofluorescence analysis of L929 cells using RPS25 antibody (CAB15314) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using RPS25 antibody (CAB15314) at dilution of 1:100. Blue: DAPI for nuclear staining.