Cell Biology Antibodies 1

Anti-RPS20 Antibody (CAB10363)

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SKU:
CAB10363
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-RPS20 Antibody
Antibody SKU:CAB10363
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-119 of human RPS20 (NP_001014.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, HT-1080, Mouse kidney, Mouse liver, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-119 of human RPS20 (NP_001014.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAFK DTGK TPVE PEVA IHRI RITL TSRN VKSL EKVC ADLI RGAK EKNL KVKG PVRM PTKT LRIT TRKT PCGE GSKT WDRF QMRI HKRL IDLH SPSE IVKQ ITSI SIEP GVEV EVTI ADA
Gene ID:6224
Uniprot:P60866
Cellular Location:Cytoplasm
Calculated MW:13kDa/16kDa
Observed MW:15kDa
Synonyms:RPS20, S20
Background:Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the small nucleolar RNA gene U54, which is located in its second intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Two transcript variants encoding different isoforms have been identified for this gene.
UniProt Protein Function:RPS20: a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the small nucleolar RNA gene U54, which is located in its second intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Apr 2009]
UniProt Protein Details:

Protein type:Ribosomal; Translation

Chromosomal Location of Human Ortholog: 8q12.1

Cellular Component: cytosol; extracellular matrix; membrane; nucleoplasm

Molecular Function:protein binding; RNA binding; structural constituent of ribosome

Biological Process: mRNA catabolic process, nonsense-mediated decay; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translation; translational initiation; viral transcription

NCBI Summary:Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the small nucleolar RNA gene U54, which is located in its second intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Apr 2009]
UniProt Code:P60866
NCBI GenInfo Identifier:46397703
NCBI Gene ID:6224
NCBI Accession:P60866.1
UniProt Secondary Accession:P60866,P17075, Q5M8S9, B2R4F4, B4DW28,
UniProt Related Accession:P60866
Molecular Weight:13kDa
NCBI Full Name:40S ribosomal protein S20
NCBI Synonym Full Names:ribosomal protein S20
NCBI Official Symbol:RPS20  
NCBI Official Synonym Symbols:S20  
NCBI Protein Information:40S ribosomal protein S20
UniProt Protein Name:40S ribosomal protein S20
UniProt Synonym Protein Names:Small ribosomal subunit protein uS10
Protein Family:40S ribosomal protein
UniProt Gene Name:RPS20  
Anti-RPS20 Antibody (CAB10363)Western blot analysis of extracts of various cell lines, using RPS20 antibody (CAB10363) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-RPS20 Antibody (CAB10363)Immunofluorescence analysis of L929 cells using RPS20 antibody (CAB10363) at dilution of 1:100. Blue: DAPI for nuclear staining.
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