Cell Biology Antibodies 7
Anti-RPS17 Antibody (CAB16426)
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- SKU:
- CAB16426
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-RPS17 Antibody |
| Antibody SKU: | CAB16426 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPS17 (NP_001012.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, A-549, DU145, BxPC-3, NIH/3T3, mouse brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPS17 (NP_001012.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGRV RTKT VKKA ARVI IEKY YTRL GNDF HTNK RVCE EIAI IPSK KLRN KIAG YVTH LMKR IQRG PVRG ISIK LQEE ERER RDNY VPEV SALD QEII EVDP |
| Gene ID: | 6218 |
| Uniprot: | P08708 |
| Cellular Location: | |
| Calculated MW: | 15kDa |
| Observed MW: | 20kDa |
| Synonyms: | RPS17, DBA4, RPS17L, RPS17L1, RPS17L2, S17 |
| Background: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S17E family of ribosomal proteins and is located in the cytoplasm. Mutations in this gene cause Diamond-Blackfan anemia 4. Alternative splicing of this gene results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. |
| NCBI Summary: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S17E family of ribosomal proteins and is located in the cytoplasm. Mutations in this gene cause Diamond-Blackfan anemia 4. Alternative splicing of this gene results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Apr 2014] |
| UniProt Code: | P08708 |
| NCBI GenInfo Identifier: | 4506693 |
| NCBI Gene ID: | 6218 |
| NCBI Accession: | NP_001012.1 |
| UniProt Secondary Accession: | P08708,P0CW22, B2R4U4, |
| UniProt Related Accession: | P08708 |
| Molecular Weight: | 14 |
| NCBI Full Name: | 40S ribosomal protein S17 |
| NCBI Synonym Full Names: | ribosomal protein S17 |
| NCBI Official Symbol: | RPS17 |
| NCBI Official Synonym Symbols: | S17; DBA4; RPS17L; RPS17L1; RPS17L2 |
| NCBI Protein Information: | 40S ribosomal protein S17 |
| UniProt Protein Name: | 40S ribosomal protein S17 |
| UniProt Synonym Protein Names: | Small ribosomal subunit protein eS17 |
| UniProt Gene Name: | RPS17 |
 | Western blot analysis of extracts of various cell lines, using RPS17 antibody (CAB16426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of L929 cells using RPS17 antibody (CAB16426) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using RPS17 antibody (CAB16426) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using RPS17 antibody (CAB16426) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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